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Showing: 4651 - 4700 of 171784 results

  2. ARK2aa

    Plasmid
    #81013
    Purpose
    E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)
    Depositor
    Taek Soon Lee
    Article
    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10
    Inserts
    MevTo-BBa1002-pTrc-Mkco-aphA
    and PMD
    Promoter
    LacUV5 and Trc promoter
    Available Since
    Aug. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    81013-plasmid-map-sequence-id-153394

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  19. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Available Since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

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  24. pHAGE-cDEST-APEX2

    Plasmid
    #214993
    Purpose
    Gateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    APEX2-Flag
    Expression
    Mammalian
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    214993-plasmid-map-sequence-id-443330

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  32. GPR19-DuET

    Plasmid
    #213274
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR19 (GPR19 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213274-plasmid-map-sequence-id-430821

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  34. TFORF1335

    Plasmid
    #142786
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU3F2 (POU3F2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142786-plasmid-map-sequence-id-395849

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  47. Protein2PAM_NmeN4C.1

    Plasmid
    #250218
    Purpose
    Express Protein2PAM_NmeN4C.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4C.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 E954C/Q981G/F1020Y/A1021S/C1023L/H1024D/…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250218-plasmid-map-sequence-id-501771

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  48. Protein2PAM_NmeN4G.1

    Plasmid
    #250224
    Purpose
    Express Protein2PAM_NmeN4G.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/V980K/Q981A/Q989T/N996E/S100…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250224-plasmid-map-sequence-id-501770

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  49. pLEXm-hCD45d

    Plasmid
    #247052
    Purpose
    For mammalian expression of human CD45 domains 1 and 2 with C-terminal Maltose-Binding Protein fusion for secretion into the medium
    Depositor
    Omer Dushek
    Article
    Vankayala et al Bioconjug Chem. 2026 Feb 18;37(2):281-29
    Insert
    hCD45d1d2-MBP
    Tags
    His6 and Maltose binding protein (MBP)
    Expression
    Mammalian
    Mutation
    Domains 1 and 2 only, corresponding to residues 2…
    Promoter
    chick β-actin promoter
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    247052-plasmid-map-sequence-id-492518

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Showing: 4651 - 4700 of 171784 results