220,728 results

1. pCMV6M-PAK1 H83L H86L K299R

   Plasmid

   #26592

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   H83L H86L K299R Dominant negative

   Promoter

   Available since

   March 9, 2011

   Availability

   Academic Institutions and Nonprofits only

2. TFORF1481

   Plasmid

   #141827

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TP63 (TP63 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SQ171/pRibo-T

   Plasmid

   #69342

   Purpose

   SQ171 strain supported by pRibo-T plasmid

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   Ribo-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SQ171/pRibo-T

   Plasmid

   #69342

   Purpose

   SQ171 strain supported by pRibo-T plasmid

   Depositor

   Michael Jewett

   Article

   Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

   Insert

   Ribo-T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYES2.1-TRP1

   Plasmid

   #11007

   Depositor

   Francisco Malagon

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 without stop codon

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYES2.1-TRP1

   Plasmid

   #11007

   Depositor

   Francisco Malagon

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 without stop codon

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0531

   Plasmid

   #143039

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PAX6 (PAX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSR348

    Plasmid

    #124713

    Purpose

    Expression of narX under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narX (narX )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S193* - LacI is truncated and likely deactivated.…

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0068

    Plasmid

    #142444

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF451 (ZNF451 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ3H-Mst2 K56R

    Plasmid

    #12206

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Catalytically inactive: K56R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJ3H-Mst2 K56R

    Plasmid

    #12206

    Depositor

    Jonathan Chernoff

    Article

    Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Catalytically inactive: K56R

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENN.AAV.CMVs.Pl.Cre.rBG (AAV1)

    Viral Prep

    #105537-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CMV

    Tags

    None

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6M FKBP12

    Plasmid

    #194122

    Purpose

    Expresses FKBP12 protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    FKBP1A (FKBP1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF0872

    Plasmid

    #141743

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXB8 (HOXB8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ptet

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ptet-RFP-shR-rtTA

    Plasmid

    #35625

    Purpose

    Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

    Depositor

    Howard Gu

    Article

    Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

    Type

    Empty backbone

    Use

    AAV and RNAi; Tet inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ptet

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

    Viral Prep

    #174007-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Promoter

    Syn

    Tags

    None

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

24. mRuby-C1

    Plasmid

    #54552

    Purpose

    Localization: C1 Cloning Vector, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. mRuby-C1

    Plasmid

    #54552

    Purpose

    Localization: C1 Cloning Vector, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pHygG2

    Plasmid

    #200066

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXY027

    Plasmid

    #98915

    Purpose

    Express FtsZ tagged with GFP in E coli

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    FtsZ
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-DIO-EGFP (AAV8)

    Viral Prep

    #50457-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

29. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF2470

    Plasmid

    #142097

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PDX1 (PDX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid

    #135263

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. QUE37C/pLBS.CAG

    Plasmid

    #129340

    Purpose

    ATP measurement, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRDA_174

    Plasmid

    #136476

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    EnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187922

    Purpose

    Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX2T merlin 1-595

    Plasmid

    #11629

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX2T merlin 1-595

    Plasmid

    #11629

    Depositor

    Vijaya Ramesh

    Article

    Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CRY2olig-PR

    Plasmid

    #218917

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-PR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CRY2olig-GR

    Plasmid

    #218919

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

    Depositor

    Tatyana Shelkovnikova

    Article

    Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

    Insert

    CRY2olig-mCherry tagged poly-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep

    #100833-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Tags

    None

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

43. TFORF0273

    Plasmid

    #142580

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF3 (TCF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Watermelon

    Pooled Library

    #155257

    Purpose

    Lineage tracing library

    Depositor

    Aviv Regev, Joan Brugge

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Expression

    Mammalian

    Use

    Lentiviral

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

46. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDONRp5-p2 GFP-NShroom3-iLID

    Plasmid

    #170976

    Purpose

    N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    NShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    eGFP and iLID

    Expression

    Mutation

    From isoform 2, deleted amino acids 1389 - 1805,…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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