20,421 results
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Plasmid#61621PurposeNon-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activityDepositorInsertAKAR4-Kras
UseTagsC-terminal targeting sequence from Kras, Cerulean…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 5F-L YFP
Plasmid#84914PurposeMammalian expresion of TDP-43 5F-L YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationF147L, F149L, F194L, F229L, F231LPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1221YC
Plasmid#61705PurposeGateway compatible BiFC vector (pEarleyGate202-YC)DepositorTypeEmpty backboneUseBifc assayTagsYFP-C and FLAGExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHisExpressionBacterialMutationPromoterPgracAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHL-avitag3
Plasmid#99847Purposemammalian expression with secretive signal and avi tag for biotinylationDepositorInsertephrin b2 (EPHB2 Human)
UseTagsAviExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-lambda red operon
Plasmid#165521PurposeOperon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.DepositorInsertlambda red operon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
YPet-N1
Plasmid#54637PurposeLocalization: N1 Cloning Vector, Excitation: 513, Emission: 527DepositorTypeEmpty backboneUseTagsYPetExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEV53D
Plasmid#44168DepositorInsertEquine infectious anemia virus (EIAV) gag-pol,rev, and S2
UseLentiviralTagsExpressionMammalianMutationA131T mutation in gag; Deletion of viral LTR sequ…PromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-24xGCN4_v4-kif18b-24xPP7
Plasmid#74928PurposeTranslation reporterDepositorInsert24xGCN4_v4-kif18b-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL204
Plasmid#22282PurposeExpresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membraneDepositorInsertpCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#2
Plasmid#64246Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK391 (mAID-BSD)
Plasmid#121192PurposeC-terminal taggingDepositorInsertmAID-BSD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Klf2
Plasmid#50786Purposeretroviral expression of mouse Klf2DepositorInsertKlf2 (Klf2 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
UseTagsSUMOExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kindlin2
Plasmid#105305PurposeTo study effect of Kindlin 2 on Cell/ECM adhesionDepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a1SE
Plasmid#49168PurposepHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
UseTagsmyc and pHGFP (pHIuorin)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyclin E-pBI-EGFP
Plasmid#16654DepositorInsertCyclin E (CCNE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Neurod1
Plasmid#30129DepositorInsertNeuroD1 (Neurod1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB-EF1a-NLuc-IRES-Puro
Plasmid#130936PurposePiggyBac vector for constitutive NanoLuc expression in mammalian cellsDepositorInsertNanoLuc
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-H3.3-polyA
Plasmid#59780PurposeExpress Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertH3F3B (H3-3B Human)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A
Pooled Library#92385PurposeCrispriDepositorHas ServiceLentiviral PrepUseLentiviralAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MetGFP
Plasmid#37560DepositorInsertMET (MET Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CD63-L7Ae
Plasmid#180503PurposeHuman CD63-L7Ae fusion proteinDepositorInsertHuman CD63-L7Ae fusion protein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-HaloTag (w876-1)
Plasmid#29644DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseEntry vectorTagsHaloTagExpressionMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GINKO2
Plasmid#177116PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2DepositorInsertGINKO2
UseTags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(Q61L)
Plasmid#13720DepositorInsertEGFP-Rac1(Q61L) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PRDM16
Plasmid#15503DepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro TPR-Met
Plasmid#10902DepositorUseRetroviralTagsExpressionMammalianMutationConstitutively active form of the c-Met receptorPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO GFP-MDC1 (779-2)
Plasmid#26285DepositorInsertMediator of DNA damage checkpoint 1 (MDC1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFBDM
Plasmid#110738PurposePlasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transpositionDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP
Plasmid#47904PurposeAn AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.DepositorInserteNpHR3.0
UseAAVTagsiRFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-FLAG (w210-2)
Plasmid#17423PurposeGateway entry vector for N-term FLAG tagDepositorTypeEmpty backboneUseEntry vectorTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorInsertAKAR4-NES
UseTagsCerulean, NES, and cpVenus[E172]ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)
Viral Prep#112677-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJG100
Plasmid#138966PurposePeft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegansDepositorInsertwrmScarlet1-10
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKC alpha WT
Plasmid#21232DepositorInsertPKC alpha (PRKCA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRB-LgBiT
Plasmid#182650PurposeFRB-LgBiTDepositorInsertFRB-LgBiT fusion
UseTagsLgBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV MCL1 Puro
Plasmid#140746PurposeExpresses MCL1 in mammalian cellsDepositorInsertMCL1 (MCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorInsertMS2-P65-HSF1 (HSF1 Human, Synthetic)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LKB1
Plasmid#8590DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only