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164,515 results

Showing: 6801 - 6850 of 164515 results

  1. pDONR223_S100A13_WT_V5

    Plasmid
    #82975
    Purpose
    Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    David Root, Matthew Meyerson, Jesse Boehm
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    S100A13 (S100A13 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82975-plasmid-map-sequence-id-162776

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  2. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  3. pSPPac-hp

    Plasmid
    #48123
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):
    Insert
    signal peptide of Pac
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48123-plasmid-map-sequence-id-69950

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  8. IL1RN gRNA3

    Plasmid
    #113132
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113132-plasmid-map-sequence-id-224944

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  10. CMV HaloTag-LgBiT Vector

    Plasmid
    #236917
    Purpose
    Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines
    Depositor
    Promega Corporation
    Has Service
    DNA
    Type
    Empty backbone
    Use
    Tags
    HaloTag (R)-LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236917-plasmid-map-sequence-id-474757

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  11. pNL [NlucP/CEBP-RE/Hygro] Vector

    Plasmid
    #236832
    Purpose
    Express CEBP response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CEBP (CEBPA Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236832-plasmid-map-sequence-id-471833

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  12. pGL4 [luc2P/ATF4-RE/Hygro] Vector

    Plasmid
    #236816
    Purpose
    Express ATF4 response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ATF4 (ATF4 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    None
    Promoter
    minP
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236816-plasmid-map-sequence-id-471822

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  13. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Article
    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, H1, and U6
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  18. CT691

    Plasmid
    #233660
    Purpose
    BLADE (two-input, four-output decoder); Cre excises lox sites to express GFP, Flp excises FRT sites to express iRFP, and the presence of both recombinases is designed to express mRuby.
    Depositor
    Wilson Wong
    Article
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Insert
    BLADE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233660-plasmid-map-sequence-id-464957

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  20. TFORF1533

    Plasmid
    #143822
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZBTB41 (ZBTB41 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143822-plasmid-map-sequence-id-471135

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  29. pSCL758

    Plasmid
    #219504
    Purpose
    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219504-plasmid-map-sequence-id-435244

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  30. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  37. pEGFP-N3-FLuc-3BoxB_O

    Plasmid
    #147078
    Purpose
    Mammalian Expression of 3BoxB
    Depositor
    Elisa Izaurralde
    Insert
    3BoxB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147078-plasmid-map-sequence-id-289525

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  38. pEGFP-N3-FLuc-7BoxB_O

    Plasmid
    #147080
    Purpose
    Mammalian Expression of 7BoxB
    Depositor
    Elisa Izaurralde
    Insert
    7BoxB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147080-plasmid-map-sequence-id-289527

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  39. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  40. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

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  41. TFORF0234

    Plasmid
    #142487
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MNX1 (MNX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    142487-plasmid-map-sequence-id-353638

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  43. pEGFP-N3-FLuc-5BoxB_O

    Plasmid
    #147079
    Purpose
    Mammalian Expression of 5BoxB
    Depositor
    Elisa Izaurralde
    Insert
    5BoxB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    147079-plasmid-map-sequence-id-355330

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  46. IL1RN gRNA4

    Plasmid
    #113133
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, Synthetic Biology, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113133-plasmid-map-sequence-id-226956

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  47. IL1RN gRNA2

    Plasmid
    #113131
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113131-plasmid-map-sequence-id-224942

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Showing: 6801 - 6850 of 164515 results