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Showing: 5481 - 5520 of 219936 results

  2. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (AAV1)

    Viral Prep
    #68719-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (#68719). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor with bicistronic mRuby2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tobias Rose, Tobias Bonhoeffer, Mark Huebener
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Promoter
    CAG-FLEX
    Tags
    mRuby2 (Cre-dependent)
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  7. Matrix-roGFP

    Plasmid
    #49437
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    Matrix-roGFP2
    Use
    Adenoviral
    Tags
    Cytochrome c oxidase subunit IV mitochondrial tar…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49437-plasmid-map-sequence-id-257693

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  8. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  13. AAV phSyn1(S)-tdTomato-WPRE (AAV5)

    Viral Prep
    #51506-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV phSyn1(S)-tdTomato-WPRE (#51506). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-tdTomato-WPRE plasmid DNA. hSyn-driven tdTomato expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    phSyn1
    Tags
    tdTomato
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)

    Viral Prep
    #100043-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  18. AAV-CAG-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #179254-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CAG
    Tags
    None
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only

  19. pLKO.3G

    Plasmid
    #14748
    Depositor
    Diane Mathis, Christophe Benoist
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  23. pAAV-nEF-Con/Foff DREADD Gi-mCherry (AAV8)

    Viral Prep
    #177673-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff DREADD Gi-mCherry (#177673). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff DREADD Gi-mCherry plasmid DNA. nEF-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition only in Cre expressing cells and not in Flp expressing cells. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    nEF
    Tags
    mCherry
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  26. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  27. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  31. pCMV-15F11-HA-mEGFP

    Plasmid
    #129590
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129590-plasmid-map-sequence-id-254270

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  34. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid
    #29655
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, GST, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29655-plasmid-map-sequence-id-196920

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  37. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep
    #98747-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Nathaniel Heintz, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  39. pComb3XSS

    Plasmid
    #63890
    Purpose
    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Inserts
    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    Use
    Phage display
    Tags
    6x His, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63890-plasmid-map-sequence-id-449948

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Showing: 5481 - 5520 of 219936 results