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171,789 results

Showing: 7851 - 7900 of 171789 results

  5. pdCas9-humanized

    Plasmid
    #44246
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dead Cas9 with 3X NLS
    Use
    CRISPR and Retroviral; Vector
    Tags
    3x NLS
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44246-plasmid-map-sequence-id-60247

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  10. CMV-Y-GECO1

    Plasmid
    #55766
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Tags
    6x His tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55766-plasmid-map-sequence-id-89442

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  12. pADH100

    Plasmid
    #90980
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90980-plasmid-map-sequence-id-180507

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  15. pRK5F-ALK5-HA(ecto)

    Plasmid
    #31720
    Depositor
    Rik Derynck
    Insert
    ALK5 (TGFBR1 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Available Since
    Feb. 14, 2012
    Availability
    Academic Institutions and Nonprofits only

  23. DsRed1 Munc13-4

    Plasmid
    #245001
    Purpose
    Distribution and localization of human wild-type Munc13-4 in cells
    Depositor
    Thomas Martin
    Insert
    UNC13D (UNC13D Human)
    Tags
    DsRed1
    Expression
    Mammalian
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    245001-plasmid-map-sequence-id-488316

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  26. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Kevin McGowan
    Insert
    Sec61 (SEC61B Human)
    Tags
    HaloTag7
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    123285-plasmid-map-sequence-id-242690

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  29. HTR2B-DuET

    Plasmid
    #213322
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2B (HTR2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213322-plasmid-map-sequence-id-430858

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  32. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  34. pKL2299

    Plasmid
    #186332
    Purpose
    This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.
    Depositor
    Kan Wang
    Article
    Kang et al Front Plant Sci. 2022 May 4;13:860971. d
    Insert
    virulence genes from Agrobacterium tumefaciens pTiBo542
    Expression
    Bacterial
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    186332-plasmid-map-sequence-id-374264

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  36. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Promoter
    Custom
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  40. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  45. DsRed2-Mito-7

    Plasmid
    #55838
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    DsRed2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55838-plasmid-map-sequence-id-244346

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  46. CMV-GFP-Kir2.1

    Plasmid
    #107184
    Purpose
    mammalian expression of Kir2.1
    Depositor
    Bikash Pattnaik
    Insert
    KCNJ2 (KCNJ2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    107184-plasmid-map-sequence-id-203927

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  47. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98998-plasmid-map-sequence-id-190366

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  48. pUC57-mitoLbNOX

    Plasmid
    #74448
    Purpose
    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    mitoLbNOX
    Tags
    Flag tag with linker and mitochondrial targeting …
    Mutation
    Human codon optimized
    Available Since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    74448-plasmid-map-sequence-id-203966

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  49. pLV_Zika_NS3_Flag

    Plasmid
    #79635
    Purpose
    Third generation Self-inactivating lentivector expressing NS3 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS3
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    79635-plasmid-map-sequence-id-152697

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  50. pAcGFP-N1_CBP

    Plasmid
    #221903
    Purpose
    Transient expression of GFP-tagged CBP in mammalian cells
    Depositor
    Daniel Bose
    Insert
    CBP (CREBBP Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N/A
    Available Since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    221903-plasmid-map-sequence-id-437447

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Showing: 7851 - 7900 of 171789 results