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169,450 results

Showing: 7851 - 7900 of 169450 results

  5. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  8. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Available Since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  13. pBUE411-GGB

    Plasmid
    #198233
    Purpose
    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411
    Depositor
    Andrea Gallavotti
    Article
    Chen et al bioRxiv 2022.09.02.506370
    Inserts
    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    Use
    CRISPR; Plant binary vector
    Expression
    Plant
    Promoter
    Ubiquitin and maize PLTP
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198233-plasmid-map-sequence-id-495715

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  17. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid
    #221359
    Purpose
    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221359-plasmid-map-sequence-id-436820

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  27. pLenti4/TO-hEPO

    Plasmid
    #50437
    Purpose
    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
    Depositor
    Juan Melero-Martin
    Article
    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:
    Insert
    EPO (EPO Human)
    Use
    Lentiviral; Tetracycline-regulatable (t-rex)
    Expression
    Mammalian
    Promoter
    CMV/TO
    Available Since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid
    #135267
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135267-plasmid-map-sequence-id-269144

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  35. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep
    #162379-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  39. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Article
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  43. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  44. TFORF1618

    Plasmid
    #144295
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NF1 (NF1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144295-plasmid-map-sequence-id-353411

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  46. TBXA2R-Tango

    Plasmid
    #66517
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TBXA2R (TBXA2R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. RHPMP132 (mEOS3.1-beta Actin)

    Plasmid
    #88836
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mEOS3.1
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Tags
    mEOS3.1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88836-plasmid-map-sequence-id-177523

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Showing: 7851 - 7900 of 169450 results