Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,538 results

Showing: 7851 - 7900 of 179538 results

  6. AAVS1-Puro CAG FUCCI

    Plasmid
    #136934
    Purpose
    donor plasmid for constitutive FUCCI expression in human cells
    Depositor
    Xiaoping Bao
    Publication
    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    Use
    Synthetic Biology
    Tags
    Clover and mKO2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    136934-plasmid-map-sequence-id-269815

    Enlarge

  11. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid
    #127366
    Purpose
    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    127366-plasmid-map-sequence-id-246342

    Enlarge

  14. hFerritinH

    Plasmid
    #122652
    Purpose
    Expression of hFerritinH in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Expression
    Bacterial
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122652-plasmid-map-sequence-id-237549

    Enlarge

  20. SMARCA2-GFP

    Plasmid
    #65390
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMARCA2 (SMARCA2 Human)
    Tags
    EmGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    65390-plasmid-map-sequence-id-306090

    Enlarge

  26. pcDNA3-CXCL12-sfGFP

    Plasmid
    #98961
    Purpose
    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCL12 (CXCL12 Human)
    Tags
    Superfolder GFP
    Expression
    Mammalian
    Mutation
    Full-length, wildtype
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    98961-plasmid-map-sequence-id-193141

    Enlarge

  28. pQUAST-attB

    Plasmid
    #104880
    Purpose
    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Expression
    Insect
    Available since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    104880-plasmid-map-sequence-id-204757

    Enlarge

  29. GoldenPiCS Kit

    Plasmid kit
    #1000000133
    Purpose
    A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications.
    Depositor
    Brigitte Gasser
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid
    #106174
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has service
    AAV1 and AAV9
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Mutation
    GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    106174-plasmid-map-sequence-id-204113

    Enlarge

  31. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    105921-plasmid-map-sequence-id-443051

    Enlarge

  32. phage UbiC NLS HA stdMCP stdHalo

    Plasmid
    #104999
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
    Depositor
    Jeffrey Chao
    Publication
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    stdMCP-stdHalo
    Use
    Lentiviral
    Tags
    HA tag, NLS, Two synonymous copies of the Halo-ta…
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    104999-plasmid-map-sequence-id-202896

    Enlarge

  33. pEGFP-N-Drd1

    Plasmid
    #104358
    Purpose
    Expresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.
    Depositor
    Kirk Mykytyn
    Publication
    Domire et al Cell Mol Life Sci. 2011 Sep;68(17):2951-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dopamine receptor D1 (Drd1) (Drd1 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    104358-plasmid-map-sequence-id-200988

    Enlarge

  37. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid
    #89351
    Purpose
    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K607R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    89351-plasmid-map-sequence-id-178227

    Enlarge

  39. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid
    #84446
    Purpose
    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Publication
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has service
    AAV1, AAV5, and AAV8
    Insert
    Jaws-KGC-tdTomato-ER2
    Use
    AAV
    Tags
    ER2, KGC, and tdTomato
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    84446-plasmid-map-sequence-id-317050

    Enlarge

  43. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    83961-plasmid-map-sequence-id-214594

    Enlarge

  44. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #85008
    Purpose
    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-ires-tTA
    Expression
    Mammalian
    Available since
    Nov. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    85008-plasmid-map-sequence-id-167314

    Enlarge

  45. pFUSEss-CHIg-mG1_M18

    Plasmid
    #82357
    Purpose
    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    82357-plasmid-map-sequence-id-157227

    Enlarge

  49. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73440-plasmid-map-sequence-id-135339

    Enlarge

  50. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71657-plasmid-map-sequence-id-422687

    Enlarge

Showing: 7851 - 7900 of 179538 results