178,225 results

Showing: 7851 - 7900 of 178225 results

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TIGRE docking site targeting vector

Plasmid

#61580

Purpose

Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Type

Empty backbone

Use

Mouse Targeting

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt3

Plasmid

#66832

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt3 (ESYT3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-TORCAR

Plasmid

#64930

Purpose

Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

Lyn-TORCAR (EIF4EBP1 Human, Synthetic)

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-mRuby2-FRET-10

Plasmid

#58169

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

Clover and mRuby2

Expression

Mammalian

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pPHT-PIP2

Plasmid

#60937

Purpose

Fluorescent FPX sensor for PIP2

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PIP2 dimerization dependent sensor RA+B monomers

Tags

dimerization dependent fluorescent protein monome…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pKvenus-Scribble

Plasmid

#58738

Purpose

Expresses Venus-tagged human Scribble protein

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

Scribble (SCRIB Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-H3-23

Plasmid

#55058

Purpose

Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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TRPC3-Myc

Plasmid

#25902

Depositor

Craig Montell

Article

Li et al Neuron. 1999 Sep . 24(1):261-73.

Insert

TRPC3 (Trpc3 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mCitrine-C1

Plasmid

#54587

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

mCitrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50464

Purpose

Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

Depositor

Bryan Roth

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Promoter

human Synapsin 1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Tags

His

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pRcCMV Cep215 (Nigg CW493)

Plasmid

#41152

Depositor

Erich Nigg

Article

Graser et al J Cell Sci. 2007 Dec 15;120(Pt 24):4321-

Insert

Cep215 (CDK5RAP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-tdTomato-SpHis5

Plasmid

#44640

Purpose

Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection

Depositor

Kurt Thorn

Insert

tdTomato

Expression

Yeast

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-hTRAF3

Plasmid

#44032

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

S393G

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pLHCX-FLAG-mPKM2

Plasmid

#44239

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M2 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2

Plasmid

#44104

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBID-UASC

Plasmid

#35200

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pPROBE-NT

Plasmid

#37818

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG25

Plasmid

#35121

Depositor

John McCusker

Article

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

natMX4

Use

Yeast replicating

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCDNsam-Hnf4a

Plasmid

#33002

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMX-Gal4-TEAD3

Plasmid

#33106

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD3 (TEAD3 Human)

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-TEAD1

Plasmid

#33108

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD1 (TEAD1 )

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG:H2B-EGFP

Plasmid

#32599

Depositor

Anna-Katerina Hadjantonakis, Virginia Papaioannou

Article

Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

Insert

H2B-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRETightBI-RY-0

Plasmid

#31463

Depositor

Phil Sharp

Article

Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

Insert

eYFP and mCherry

Tags

NLS

Expression

Mammalian

Available Since

Jan. 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-YFP-PKD1

Plasmid

#31527

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-HA-Protein Kinase D (PRKD1 Human)

Tags

HA and YFP

Expression

Mammalian

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
CHMP6_GFP

Plasmid

#31806

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP6 (CHMP6 Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET MBP mCherry LIC cloning vector (MBP-mCherry)

Plasmid

#29747

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 IKKE K38A flag

Plasmid

#26202

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon K38A (IKBKE Human)

Tags

Flag

Expression

Mammalian

Mutation

Catalytic Lysine changed to Alanine (K38A)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEC22

Plasmid

#30181

Depositor

Lalita Ramakrishnan

Article

Takaki et al Cell Rep. 2012 Jul 26;2(1):175-84. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdKatuschka2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5B-FRT-GFP-αTAT1[D157N]

Plasmid

#27100

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

αTubulin K40 acetyltransferase (ATAT1 Human)

Tags

GFP and S-tag

Expression

Mammalian

Mutation

D157N

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
p4xDRE-Luc

Plasmid

#16534

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

4xDRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

May 12, 2010

Availability

Academic Institutions and Nonprofits only
pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
SFG.wtCNa_opt.IRES.eGFP

Plasmid

#22489

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

codon optimised wild type Calcineurin A (PPP3CA Human)

Use

Retroviral

Mutation

Codon Optimised wtCNa

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
SA-betageo

Plasmid

#21709

Depositor

Philippe Soriano

Article

Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

Insert

SAbgeo

Use

Mouse gene trapping

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-miR21

Plasmid

#20381

Depositor

Bryan Cullen

Article

Zeng et al RNA. 2003 Jan . 9(1):112-23.

Insert

miR21 (MIR21 Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PKC beta I DN

Plasmid

#16381

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL hygro 12S E1A

Plasmid

#18748

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
human gamma-catenin pcDNA3

Plasmid

#16827

Depositor

Eric Fearon

Article

Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

Insert

gamma-catenin (JUP Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc RhoA wt

Plasmid

#15899

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBABE-puro LAP2

Plasmid

#15712

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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