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179,553 results

Showing: 11001 - 11050 of 179553 results
  1. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid
    #105714
    Purpose
    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
    Depositor
    Has service
    AAV8
    Insert
    GCaMP6s
    Use
    AAV; Flp/frt
    Expression
    Mammalian
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pDEST14-Cys-SpyCatcher002

    Plasmid
    #102829
    Purpose
    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
    Depositor
    Insert
    Cys-SpyCatcher002
    Tags
    GSSGS, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Cysteine residue in N-terminal region
    Promoter
    T7
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  6. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  7. CMV Marina-T2A-nls-mCherry

    Plasmid
    #74216
    Purpose
    genetically-encoded fluorescent voltage indicator
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    fluorescent protein voltage sensor
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pLV_Zika_Flag_NS4A

    Plasmid
    #79636
    Purpose
    Third generation Self-inactivating lentivector expressing NS4A from Zika virus
    Insert
    NS4A
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  9. pG1AK-sfGFP

    Plasmid
    #71739
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Insert
    sfGFP
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  11. pGolt-mCherry / pGolt3-mCherry

    Plasmid
    #73297
    Purpose
    Visualization of Dendritic Golgi Satellite
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    Tags
    ER export and mCherry
    Expression
    Mammalian
    Promoter
    CMV immediate early promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  12. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Mutation
    changed Asp at position 249 + 250 to Val
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 11001 - 11050 of 179553 results