20,802 results
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Plasmid#22011PurposeMammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assaysDepositorInsertVC155
TagsHAExpressionMammalianMutationChanged Alanine 206 to LysineAvailable SinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT2T3
Plasmid#50591PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, U6-29t plus, U6-1p, T3
UseCRISPR; Pcr templateExpressionPlantAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro DEST (w529-2)
Plasmid#19068Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
L4440
Plasmid#1654DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAiTagsT7p, T7p, lacZN, OriF1>>, OriF1<<ExpressionWormAvailable SinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
TagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialPromoterT7Available SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0046-EF1a-PspCas13b-NES-HIV
Plasmid#103862PurposeExpresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-hspDRv7_scGstlt
Plasmid#108870PurposescGESTALT V7 lineage barcode for zebrafishDepositorInsertscGESTALT lineage tracing target V7
UseTol2 integration into zebrafish genomeAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tdTomato
Plasmid#83029Purposeexpresses tdTomato fluorescent protein under the CAG promoterDepositorInserttdTomato
ExpressionMammalianPromoterCAGAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-NCAPH
Plasmid#31214DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold(c4)
Plasmid#212018PurposeFor N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.DepositorInsertmStayGold(c4)
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK3071
Plasmid#219755PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertmcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
ExpressionPlantAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
cEF.tk-GFP
Plasmid#33308DepositorInsertHSV-TK/GFP
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RARE-luciferase
Plasmid#13458DepositorInsertRetinoic Acid Receptor Response Element
UseLuciferaseAvailable SinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
p21-Reporter
Plasmid#52432PurposeDNA damage inducible Red Fluorescent Protein for mammalian expressionDepositorInsertp21-p53 Tandem Response Element Promoter
TagsTurboRFPExpressionMammalianPromoter13X repeated p21-p53 response elementAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDL-hu FceRI alpha
Plasmid#8365DepositorInserthuman Fc epsilon RI alpha cDNA (FCER1A Human)
ExpressionMammalianAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SypHer
Plasmid#48250PurposepH sensorDepositorInsertSypHer
ExpressionMammalianMutationC199SPromoterCMVAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/gene
Pooled Library#83985PurposeHuman CRISPRa Pooled Library targeting membrane proteinsDepositorAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHSN6A01
Plasmid#50586PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…Promoter2×35Sp and AtU6-26pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV8)
Viral Prep#104492-AAV8PurposeReady-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-MCS
Plasmid#71783PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus
Plasmid#106078Purposeexpression in mammalian cellsDepositorInserthuman MLKL-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianPromoterDox-inducibleAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV9)
Viral Prep#107738-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed (AAV1)
Viral Prep#105669-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-MET
Plasmid#116762PurposeLentiviral expression of METDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
ExpressionBacterialAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMM-jRCaMP1b
Plasmid#127871PurposeRed-shifted fluorescent reporter for mitochondrial outer membrane Ca2+DepositorInsertOMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP
Plasmid#52675PurposeshRNA silencing plasmid with eGFP transfection markerDepositorTypeEmpty backboneUseRNAiTagsCMV-EGFPPromoterCMV and U6Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 WT B-Myb
Plasmid#25965DepositorInsertB-Myb (MYBL2 Human)
ExpressionMammalianAvailable SinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCherry
Plasmid#79130PurposeLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherryDepositorInserttBFP
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
V406 4HA-CKIe(K38R)
Plasmid#13725DepositorInsertcasein kinase 1 epsilon (CSNK1E Human)
Tags4 HAExpressionMammalianMutationkinase-inactive due to K38R mutationAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
phage-cmv-cfp-24xpp7
Plasmid#40652DepositorInsertCFP-24xPP7
UseLentiviralTagsCFPExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-KRAB
Plasmid#191314PurposepiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFPDepositorInsertsKRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3Kv-WPRE
Plasmid#132330Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3KV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIEZO1-HaloTag
Plasmid#207834PurposePIEZO1 fusion for biochemistry and live imagingDepositorAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHoss1
Plasmid#63158PurposeSuicide plasmid for efficient gene mutation in Gram-positive bacteriaDepositorInsertanti-secY-Pxyl/tetO-tetR
UseSuicide plasmid for gram-positive bacteriaAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-i_N1
Plasmid#85056PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
VSV.G
Plasmid#14888DepositorInsertVSV-G Envelope
ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV5)
Viral Prep#125712-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromotershort version of αCamKinase II promoter (0.4kb)TagsmScarletAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only