Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

173,379 results

Showing: 2201 - 2250 of 173379 results

  2. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107999-plasmid-map-sequence-id-217151

    Enlarge

  3. EGFP-H2B-6

    Plasmid
    #56436
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56436-plasmid-map-sequence-id-275092

    Enlarge

  5. pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (AAV9)

    Viral Prep
    #172923-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (#172923). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8f in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Promoter
    Syn
    Tags
    mRuby3
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available Since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

    Enlarge

  14. GPR158-Tango

    Plasmid
    #66332
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR158 (GPR158 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. LV-AR-R599A-N600A

    Plasmid
    #171221
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-R599A-N600A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-R599A-N600A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171221-plasmid-map-sequence-id-342450

    Enlarge

  18. pPtGE34

    Plasmid
    #107932
    Purpose
    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107932-plasmid-map-sequence-id-242338

    Enlarge

  27. mCherry-VASP-5

    Plasmid
    #55151
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    VASP
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55151-plasmid-map-sequence-id-424687

    Enlarge

  29. pSP64-mHCN2

    Plasmid
    #53060
    Purpose
    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Article
    Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7
    Insert
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    Use
    Xenopus oocyte expression
    Mutation
    E55G, R237H, and K283R polymorphisms compared to …
    Promoter
    SP6
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    53060-plasmid-map-sequence-id-82596

    Enlarge

  31. mEmerald-ZO1-C-14

    Plasmid
    #54316
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54316-plasmid-map-sequence-id-475374

    Enlarge

  34. AAV-EF1a-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #176756-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8f-WPRE (#176756). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8f-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-flex-taCasp3-TEVp (AAV5)

    Viral Prep
    #45580-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nirao Shah, Jim Wells
    Article
    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10
    Promoter
    EF1a
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only

  40. mEmerald-VEGFR2-N

    Plasmid
    #54298
    Purpose
    Localization: Membrane Receptor, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    VEGFR2 (KDR Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54298-plasmid-map-sequence-id-372994

    Enlarge

  42. TFORF3337

    Plasmid
    #144813
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144813-plasmid-map-sequence-id-404393

    Enlarge

  43. mEmerald-Beta-Catenin-20

    Plasmid
    #54017
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

    Enlarge

Showing: 2201 - 2250 of 173379 results