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161,697 results

Showing: 2801 - 2850 of 161697 results

  1. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.S)

    Viral Prep
    #59462-PHP.S
    Purpose
    Ready-to-use AAV PHP.S particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168125-plasmid-map-sequence-id-332869

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  11. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  12. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

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  13. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

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  16. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)

    Viral Prep
    #111568-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)
    Promoter
    Tags
    mCherry
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  17. pLenti6.2_miRFP670

    Plasmid
    #113726
    Purpose
    Expresses the fluorescent protein miRFP670 in a lentiviral backbone
    Depositor
    Vanessa LaPointe
    Insert
    miRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    113726-plasmid-map-sequence-id-218247

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  20. BRD4

    Plasmid
    #38942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38942-plasmid-map-sequence-id-348518

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  31. pGP-AAV-syn-jGCaMP8m-WPRE (AAV Retrograde)

    Viral Prep
    #162375-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-CAG-iSeroSnFR-Nlgn (AAV9)

    Viral Prep
    #128485-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31
    Promoter
    CAG
    Tags
    None
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  41. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Bradley McConnell
    Article
    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Use
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    182574-plasmid-map-sequence-id-450120

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  44. pAAV-hsyn-GRAB_rDA1m (AAV9)

    Viral Prep
    #140556-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1m (#140556). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1m plasmid DNA. Synapsin-driven expression of red fluorescent medium affinity dopamine sensor GRAB_rDA1m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 2801 - 2850 of 161697 results