We narrowed to 11,159 results for: ENA

1. pCS2-zfCSF3a

   Plasmid

   #168201

   Purpose

   Express zebrafish Csf3a, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3a (csf3a Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of A27

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170812

   Purpose

   Lentiviral vector to co-express an sasgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLyGo-Bs-4

   Plasmid

   #163142

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLyGo-Ec-4

   Plasmid

   #163130

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pL0-NbPT5bPro

   Plasmid

   #159416

   Purpose

   Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   NbPT5b Promoter
   

   Use

   Puc19-derived

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pL0-NbBCP1bPro

   Plasmid

   #159417

   Purpose

   Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   NbBCP1b Pro
   

   Use

   Puc19-derived

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLyGo-Ec-1

   Plasmid

   #163127

   Purpose

   pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Ubiquitin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168168

   Purpose

   Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-CaCyclOp(A-2x)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   E497K and C566D

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168171

   Purpose

   Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-CaCyclOp(A-2x)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLyGo-Bs-3

    Plasmid

    #163141

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YCplac33-NAT_NDT1

    Plasmid

    #140593

    Purpose

    Express NDT1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ290-D570A-SRDX

    Plasmid

    #129682

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ291-D573A-SRDX

    Plasmid

    #129683

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D573A; BthCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAWH-IRP

    Plasmid

    #133896

    Purpose

    Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLY151

    Plasmid

    #130947

    Purpose

    pspFΔHTH::λN22plus gene controlled by promoter PrhaB

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    rhaS
    pspFΔHTH::λN22plus
    

    Use

    Synthetic Biology

    Tags

    pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    The HTH domain of the pspF gene was deleted (297-…

    Promoter

    Pcon and PrhaB

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG

    Plasmid

    #127323

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)/AUU-nLuc-3XFLAG

    Plasmid

    #127305

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(+)/AAA-nLuc-3XFLAG

    Plasmid

    #127306

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.13/AUG(30)-FFLuc-3XFLAG

    Plasmid

    #127336

    Purpose

    Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+)/AGG-nLuc-3XFLAG

    Plasmid

    #127304

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1(+)/ACG-nLuc-3XFLAG

    Plasmid

    #127303

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1(+)/GGG-nLuc-3XFLAG

    Plasmid

    #127307

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGL4.13/AUG(59)-FFLuc-3XFLAG

    Plasmid

    #127337

    Purpose

    Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXW109ABS62(RS)

    Plasmid

    #123153

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS62, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXW109ABS67(RS)

    Plasmid

    #123154

    Purpose

    Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109. ParsR-ABS67, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVITRO-Fab_Theo-LUCID

    Plasmid

    #119048

    Purpose

    Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Theo-LUCID_part1
    Fab_Theo-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pVITRO-Fab_Quin-LUCID

    Plasmid

    #119049

    Purpose

    Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_Quin-LUCID_part1
    Fab_Quin-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVITRO-Fab_MTX-LUCID

    Plasmid

    #119047

    Purpose

    Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

    Inserts

    Fab_MTX-LUCID_part1
    Fab_MTX-LUCID_part2
    

    Use

    Tags

    FLAG-tag, His-tag, and Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α + CMV/SV40 enhancers and IRES

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPOR2KN0075

    Plasmid

    #117642

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Flavin-binding monooxygenase family protein {AT1G62600}(pEPOR1CB0098)and sgRNA_Flavin-binding monooxygenase family protein {AT1G62590}(pEPOR1CB0109)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEPOR1CB0109

    Plasmid

    #117571

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thaliana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Promoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62590} A. thaliana
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 (pICSL90002)

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEPOR1CB0098

    Plasmid

    #117570

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thaliana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Promoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62600} A. thaliana
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 (pICSL90002)

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTK-ENH_BsmB1-MCS-Avi

    Plasmid

    #110205

    Purpose

    Recipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice

    Depositor

    Tatjana Sauka-Spengler

    Article

    Lukoseviciute et al STAR Protoc. 2020 Jul 31;1(2):100066. do

    Insert

    ENH_BsmB1-MCS-Avi
    

    Use

    Tags

    Avi_tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTK-ENH_BsmB1-Avi-MCS-2A-Citrine

    Plasmid

    #110204

    Purpose

    Recipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Lukoseviciute et al STAR Protoc. 2020 Jul 31;1(2):100066. do

    Insert

    ENH_BsmB1-Avi-MCS-2A-Citrine
    

    Use

    Tags

    Avi_tag-2a-YFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti CRISPR V2 sgMTHFD1L_2

    Plasmid

    #106317

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET15b+2(L171C)X

    Plasmid

    #108192

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pKDsgRNA-fsaA

    Plasmid

    #102286

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    fsaA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pKDsgRNA-adhE

    Plasmid

    #102290

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    adhE gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge