31,059 results
-
Plasmid#176397PurposePlasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.DepositorInsertmScarlet-TsCC(B)
TagsmScarlet fused to TsCC(B)ExpressionBacterial and YeastPromoternoneAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
ExpressionBacterialPromoterPpro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LIC
Plasmid#62303PurposeE. coli expression vector with N-terminal Hexa-HIS and TEV cleavageDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-P43
Plasmid#112775PurposeStrong promoter activity during exponential and stationary phaseDepositorArticleInsertP43 promoter
ExpressionBacterialAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-GFP-PACT
Plasmid#105954Purposeheat shock inducible transgenesis, PACT is a centriol markerDepositorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
Plasmid#174516PurposeRecoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.DepositorInsertsPylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon
ExpressionBacterialMutationCUA anticodon, M.alvus Pyl tRNA with CGA anticodo…PromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK003
Plasmid#65110PurposeEncodes ConL1 as a Type 1 part to be used in the Dueber YTK systemDepositorInsertConL1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
TagspLacZ-EGFPExpressionBacterialPromoterpLacZAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3113 GST-p53
Plasmid#10852DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29b Tau Cys
Plasmid#108867PurposeExpresses human Tau with a mutated cysteineDepositorInsertTau (MAPT Human)
ExpressionBacterialMutationI260C C291A C322A to allow maleimide dye labellingPromoterT7Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-GFP-RGG
Plasmid#124948PurposeExpression of RGG-GFP-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbS2c-RFP
Plasmid#35329DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC-fFuCas9-HTBNLS-hph
Plasmid#121092PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 systemDepositorInsertCas9 and hygromycin resist gene
UseCRISPRExpressionBacterialPromotergpdA;trpCAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ecoli cpGFP
Plasmid#215713PurposeExpresses cpGFP in EcoliDepositorInsertcpGFP
Tags6xHisExpressionBacterialMutationcircularly permuted GFPPromoterT7Available SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T moesin 1-577
Plasmid#11637DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 WT
Plasmid#27010DepositorAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]
Plasmid#100962PurposeContains genes encoding GPP synthase, cineole synthase and IspA(S81F)DepositorInsertGPPS-CSstr-ispA*
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLAM12
Plasmid#26908DepositorInsertAcetamidase Promoter
ExpressionBacterialAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDA303
Plasmid#195199PurposeOverexpressing 6His-TetR in E. coli.DepositorInsertTetR
Tags6xHisExpressionBacterialPromoterT5 promoterAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationWTPromoterKanamycinAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 Q331K
Plasmid#27454DepositorInsertTDP-43 (TARDBP Human)
TagsFactor Xa and His6ExpressionBacterialMutationGlutamine 331 mutated to Lysine (Q331K)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQFT
Plasmid#203674PurposepQF derivative with promoter PQ5 exchanged for promoter PQJDepositorTypeEmpty backboneTags3xFLAGExpressionBacterialPromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorAvailable SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
TagsEGFPExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2A(sfGFP)
Plasmid#126964PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis APEX2-eIF4A1
Plasmid#129645PurposeExpress APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteriaDepositorInserteukaryotic translation initiation factor 4A1 (EIF4A1 Human)
Tags6xHis and APEX2ExpressionBacterialAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-FimH-9xHis
Plasmid#97305PurposeE.coli UTI89 FimH signal peptide and lectin domainDepositorInsertType 1 fimbiral adhesin FimH
Tags9xHisExpressionBacterialAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS96
Plasmid#207588PurposeGFP-TonBH20A expressionDepositorInsertGFP-TonBH20A
ExpressionBacterialMutationH20AAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS65
Plasmid#207587PurposeGFP-TonB expressionDepositorInsertGFP-TonB
ExpressionBacterialAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sc RFC5-pCDFDuet
Plasmid#239202PurposeOverexpress Sc RFC5 in E. coliDepositorInsertRFC5 (RFC5 Budding Yeast)
ExpressionBacterialAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_9C74
Plasmid#234238PurposeHeterologous protein expression of variant of super folded GFP in Escherichia coliDepositorInsert9C74
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEM8731
Plasmid#213991PurposePrpsM driven expression of mNeonGreen - sequence codon-optimized for SalmonellaDepositorInsertmNeonGreen
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
Tags3xFLAG-tag and His14-tagExpressionBacterialAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
TagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only