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Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALlR1
Plasmid#172566PurposeInducible gene expression, pACYC origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
UseTagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
BoNT/A-LC
Plasmid#31602DepositorInsertBoNT/A LC
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCF48 HLA-A*11:01
Plasmid#180449PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*11:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Q-IL6-9
Plasmid#166552PurposescFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RhoA-wt
Plasmid#12959DepositorInsertRhoA (RHOA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutH
Plasmid#86609PurposeE. coli MutH expression plasmidDepositorInsertE. coli MutH
UseTagshis-tag and ald-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUS252
Plasmid#127674PurposeExpresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbonesDepositorInsertFree Use GFP (fuGFP)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJune 24, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
Plasmid#156463PurposeExpress Heme Oxygenase (Nostocaceae) and ReBphP-PCMDepositorInsertsHeme Oxygenase
ReBphP-PCM
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97(wt)
Plasmid#14666DepositorInsertp97 (Vcp Mouse)
UseTagsRGS6xHisExpressionBacterialMutationSee depositor comments below.PromoterAvailable sinceApril 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PFO
Plasmid#92109PurposeRecombinant expression in E.ColiDepositorInsertPFO
UseTags6xHisExpressionBacterialMutationDelta27aaPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-143
Plasmid#118527PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorUseTagsGST and HisExpressionBacterialMutationPromoterT5 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP670
Plasmid#45453DepositorInsertiRFP670
UseTagsExpressionBacterialMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutS
Plasmid#86605PurposeE. coli MutS expression plasmidDepositorInsertE. coli MutS (D835R,R840E)
UseTagshis-tag and ald-tagExpressionBacterialMutationD835R, R840EPromoterT7Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
UseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7 lacAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3a aSyn murine
Plasmid#108865PurposeExpresses murine alpha synucleinDepositorInsertalpha synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
UseTagsExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RBM17_RRM
Plasmid#109786PurposeProtein expression and purification of RBM17_RRMDepositorInsertRBM17_RRM (RBM17 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mTurquoise2-EC18152
Plasmid#137074PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorInsertYcgR (ycgR )
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Delta
Plasmid#175950PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationDelta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only