32,671 results
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Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterPANI2 synthetic promoter carrying binding sites f…Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
TagsHisExpressionBacterialPromoterT7Available SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(D130N)-RagC
Plasmid#99642PurposeBacterial expression of RagA(D130N)-RagCDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
TagsGST tagExpressionBacterialPromoterT7 promoterAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 K54R
Plasmid#53170PurposeExpresses GST-HA tagged human ERK2 K54R from bacterial expression vectorDepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-acuR
Plasmid#62568PurposeAcrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Rac1 G15A
Plasmid#69355Purposeexpression of nucleotide free Rac1 in bacterial cellsDepositorAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-gusA-pBAV1k
Plasmid#30503DepositorAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
CLK2
Plasmid#39063PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
Tags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
ExpressionBacterialMutationgfp with first three leucine codons mutated to am…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH726_pJH_LldR_PlldR-sfGFP.15TAG
Plasmid#163730PurposeLactate-inducible expression of green fluorescent protein encoding fifteen amber codonsDepositorInsertlldr gfp.15TAG
ExpressionBacterialMutationgfp with first 15 leucine codons mutated to amberAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIA033
Plasmid#200420PurposethrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorTypeEmpty backboneTagsbsPAmCherryExpressionBacterialAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA034
Plasmid#200421PurposeamyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorTypeEmpty backboneTagsbsdronPAExpressionBacterialAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBC13-PGEX4T
Plasmid#25464DepositorAvailable SinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG-NHERF2
Plasmid#28292DepositorAvailable SinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22B_PcrA_2Bchimera
Plasmid#102998PurposeExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrADepositorInsertPcrA_2Bchimera
ExpressionBacterialMutationBacilllus stearothermophilus PcrA in which the 2B…PromoterT7Available SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)
Plasmid#136543PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneTagsANXA2 (23-339) and His-tagExpressionBacterialAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP1-kanMX6
Plasmid#133551PurposeYeast fluorescent (RFP) vector with PTEF promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Human ABeta-attR5
Plasmid#160436PurposeEntry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Human)
ExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-YAP-deltaC
Plasmid#21126DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
TagsEGFP-C3ExpressionBacterial and MammalianMutation5 Carboxy-terminal amino acids -FLTWL are deletedAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig1
Plasmid#64841PurposeA lentiviral vector for the expression of mouse Olig1 under doxycyline controlDepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
NEK2
Plasmid#39163PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
Tags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD33MevT
Plasmid#17814DepositorInsertMevT
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
Reb_221 aka PAS628
Plasmid#71233PurposeReb_1 with RebA(G90A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, G90A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPro24(s)-gfp
Plasmid#17813DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialMutationD234N compared to AF007834Available SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
human p53-Δ(325-355)
Plasmid#24871DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
HS-H2B-RFP
Plasmid#105953Purposeheat shock inducible transgenesisDepositorInsertH2B
TagsRFPExpressionBacterialAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPTD-DRBD
Plasmid#35622DepositorInsertPKR DRBD-1
Tags6xHIS, HA, and TATExpressionBacterialPromoterT7Available SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKEW397_AcrIIA15
Plasmid#138285PurposeExpression of AcrIIA15 proteinDepositorInsertAcrIIA15
UseCRISPRTagsMBPExpressionBacterialAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW394_AcrIIA14
Plasmid#138283PurposeExpression of AcrIIA14 proteinDepositorInsertAcrIIA14
UseCRISPRTagsSFGFPExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PTK2b
Plasmid#224237PurposeExpression of human PTK2b Kinase in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
ExpressionBacterialAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
ExpressionBacterialAvailable SinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only