5,038 results
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Plasmid#59217PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.DepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectPromoterDrosophila heat-shock promoterAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only
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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
Plasmid#73164Purposemulti-cistronic Vector for expression for Fly_FUCCI probes in insect cellsDepositorTagsmRFP1, GFPExpressionInsectMutationCyclin B Fragment aa 1-266 & E2F1 Fragment aa…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR121
Plasmid#37148DepositorAvailable SinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pUAST
Plasmid#17746DepositorAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_WT
Plasmid#162579PurposeExpresses norovirus GI.1 VP1 protein in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectPromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-frt-stop-frt-p65AD::CaM
Plasmid#64716Purposegenerating transgenic TRIC fliesDepositorInsertsp65AD::CaM
frt-stop-frt
ExpressionInsectAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-neo
Plasmid#32426DepositorInsertFlag-mCherry-T2A-GFP-T2A-neo
ExpressionInsectPromoterActin5CAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
Plasmid#52889Purpose"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expressionDepositorInsertGal4 (GAL4 Budding Yeast)
ExpressionInsectAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC153-20XUAS-IVS-B2::PEST
Plasmid#32134DepositorInsertB2::PEST
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-NS
Plasmid#149545PurposeExpression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Arp2/3_pBIG2abcd
Plasmid#173683PurposeInsect expression of the 7-subunit Human Arp2/3 complexDepositorInsertsArp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1
ExpressionInsectPromoterpolyhedrinAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIEx4-Cre
Plasmid#198280PurposeExpresses Cre in insect cellsDepositorInsertpIEx4-Cre
ExpressionInsectAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-degradALFA
Plasmid#219663PurposePermits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.DepositorInsertdegradALFA
ExpressionInsectAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2
Plasmid#59037Purposefor baculovirus expression of CAMSAP2 in Sf9 cellsDepositorAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
TagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
TagsGFP11x7 and mCherryExpressionInsectAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10
Plasmid#172918PurposeLexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7c-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ptubP-QS
Plasmid#24352DepositorInsertQS
Tagswhite geneExpressionInsectAvailable SinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
xenCGN-Myc-His
Plasmid#236505PurposeFor expression of Xenopus CGN in Insect CellsDepositorAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub
Plasmid#203966PurposeFly transformation vector with Tubulin promoter and SV40 PA site and a UTR for ubiquitous expressionDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV5B+longBRD4
Plasmid#157792PurposeExpression of long isoform of BRD4DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBPp65ADZpUw
Plasmid#26234DepositorInsertp65AD-Zip (RELA Human)
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralExpressionInsectPromoterCMV and U6 and H1Available SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorExpressionInsectPromoterpolyhedrin of AcMNPVAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
Tags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1
Plasmid#159576PurposeBaculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-DmPABPC1-V5His6_D
Plasmid#146093PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationone non-silent mutation G26S compared to the sequ…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD6
Plasmid#73915Purposetissue-specific multiplex gRNA plasmidDepositorInsert:tRNA:gRNA:tRNA:gRNA:tRNA
UseCRISPRExpressionInsectPromoterUAS-DSCPAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRExpressionInsectPromoterdU6:3Available SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP
Plasmid#32149DepositorInsertFRT> STOP STOP FRT>
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAJ075_hsCRBN
Plasmid#124214PurposeInsect cell expression vector for His6 tagged hsCRBNDepositorAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBID2_MCS>QF2
Plasmid#194963PurposeDrosophila QF2 expression under introduced sequence control, PhiC31 transgenesisDepositorTypeEmpty backboneExpressionInsectMutationQF2Available SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-GFP
Plasmid#130277PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.DepositorInsert3xP3-GFP
UseCRISPRExpressionInsectAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBPnlsLexA::p65Uw
Plasmid#26230DepositorInsertnlsLexA::p65 (RELA Human)
ExpressionInsectAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFD5_w
Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
ExpressionInsectAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-GCN4
Plasmid#78899PurposeExpresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell cultureDepositorInsertdCas9-10XGCN4
UseCRISPRTagsHAExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual LIC cloning vector (5A)
Plasmid#30121DepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBID2_UAS>MCS
Plasmid#194958PurposeDrosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesisDepositorTypeEmpty backboneExpressionInsectMutation20 x UAS sequencesAvailable SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBID2_MCS>Gal4
Plasmid#194961PurposeDrosophila Gal4 expression under introduced sequence control, PhiC31 transgenesisDepositorTypeEmpty backboneExpressionInsectMutationGal4.2Available SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL005635 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD3.1-w-dU6:3gRNA
Plasmid#123366PurposegRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).DepositorInsertU6:3-gRNA
UseCRISPRExpressionInsectAvailable SinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIB-GST-3C-CDK1
Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
TagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only