76,289 results
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Plasmid#20953Purpose3rd gen lentiviral empty control vector, PuroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only
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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES
Plasmid#124707PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NESDepositorInsertMCP_ADAR2 DD (E488Q)_NES
UseAAVTagsNESExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-humanTRAF6 (1093)
Plasmid#66929PurposeMammalian Expression of TRAF6DepositorInsertTRAF6 (TRAF6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mARG1 cassette 1
Plasmid#134343PurposeMammalian acoustic reporter gene 1 cassette 1 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX1
Plasmid#112702PurposeExpression of NFIX1 protein and GFPDepositorInsertNuclear Factor I X1 (Nfix Mouse)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mNeonGreen
Plasmid#137802PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hciap2mut
Plasmid#8339DepositorInsertciap2mut (BIRC3 Human)
UseTagsmycExpressionMammalianMutationH574APromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-47xCAG-12xMS2
Plasmid#99148PurposeEncodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert47xCAG 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3A
Plasmid#35521PurposeMammalian expression of human DNMT3A with myc tagDepositorInsertDNMT3A (DNMT3A Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F269-OKT3
Plasmid#190681PurposeEncodes an EGFRvIII-CD3 BITE protein (F269 clone)DepositorInsertAnti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA025
Plasmid#131384PurposeORFeus expression, mutantDepositorInsertORFeus
UseTagsORF2-3xFlagExpressionMammalianMutationH230A EnPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mARG1 cassette 2
Plasmid#134344PurposeMammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1
Plasmid#27099DepositorInsertα-Tubulin K40 acetyltransferase1 (ATAT1 Human)
UseTagsGFP, S-tag, and TEV siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT305 Pa01 Lenti pEF-attB LSR GFP
Plasmid#184948PurposeLentiviral delivery of recombinase landing padDepositorInsertPa01 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2a wt
Plasmid#45445DepositorInsertNR2A (Grin2a Rat)
UseTagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C
Plasmid#80932PurposeExpresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4A
Plasmid#19951DepositorInsertCUL4A (cullin 4A) (CUL4A Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215008PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP703
Plasmid#79998PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221_SLC7A8
Plasmid#132233PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A8 (SLC7A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB CAG-tdTomato
Plasmid#133569PurposeCAG tdTomato piggyBac transposonDepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA034
Plasmid#131386PurposeORFeus expression, mutantDepositorInsertORFeus
UseTagsORF2-3xFlagExpressionMammalianMutationD702Y RTPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 A775_G776insYVMA
Plasmid#40982DepositorInsertERBB2 (ERBB2 Human)
UseRetroviralTagsExpressionMammalianMutationA775_G776insYVMAPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCag SE (Self-excising) FlpO-2A-Cre EV
Plasmid#130986Purposeepisomal expression of FlpO and Cre recombinases, self excisingDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationprotamine intron added to FlpO to prevent bacteri…PromoterCMV/Chick β-actin (CAG)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorInsertVAMP 3 (VAMP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
T7_CC_Vpx-SIV_WPRE_IVT_Template
Plasmid#216792PurposeTemplate for in vitro transcription of Vpx (SIV).DepositorInsertVpx (SIV)
UseTagsExpressionMammalianMutationPromoterT7Available sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B WT
Plasmid#123092PurposeExpresses 3xFLAG-LC3B wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a
Plasmid#25030DepositorInsertDCP1a (DCP1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-EcSTH-FLAG
Plasmid#218628PurposeThis plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL_U2AF1_WT_mCherry
Plasmid#84017Purposeexpression of U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianMutationPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dyn1-pmCherryN1
Plasmid#27697DepositorInsertDynamin 1 (Dnm1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3K(1-10)
Plasmid#157993PurposeLentiviral vector of constitutive expression of mNeonGreen3K(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationG27D, D42E, M128I, K153M, T170IPromoterSFFVAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC1A2
Plasmid#131901PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC1A2 (SLC1A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p667-UBC-SCYL1-V5-miniTurbo_IDG-K
Plasmid#189911PurposeGateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertSCYL1 (SCYL1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-ALMS1
Plasmid#136828PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tagDepositorInsertSNAP-ALMS1 (ALMS1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21
Plasmid#83421PurposeMouse Rab21 (wild type)DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRPC1-FLAG
Plasmid#24408DepositorInsertTRPC1 (TRPC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE2
Plasmid#73020PurposeExpresses BE2 in mammalian cellsDepositorInsertBE2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2118
Plasmid#49143PurposeAAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.DepositorInsertssAAV-smCBA-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromotersmCBAAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
FAB(mito-LD)
Plasmid#214425PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito
UseTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-PML-IV
Plasmid#62804Purposeexpresses the isoform IV of PML (promyelocytic leukemia protein)DepositorInsertPML-IV (PML Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCD-GSDMA-N-tev-C
Plasmid#226231PurposeExpress TEVP-cleavable GSDMADepositorInsertGasdermin A (Gsdma3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSico
Plasmid#11578PurposeCre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP
Plasmid#117382PurposeAn AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
ch-TOGKDP-GFP
Plasmid#69112PurposeFor mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
UseTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only