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Mammalian Expression

76,418 results

Showing: 11201 - 11250 of 76418 results

  2. BII-Sh-FUCCI

    Plasmid
    #133363
    Purpose
    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    Clover-Geminin-IRES-mKO2-Cdt1
    Use
    Tags
    HA-tagged Clover-Geminin
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133363-plasmid-map-sequence-id-267621

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  7. hNFAT2-409:TALE:VP64:KRɸ

    Plasmid
    #160661
    Purpose
    Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.
    Depositor
    Roman Jerala
    Article
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Insert
    hNFAT2-409:TALE:VP64:KRɸ
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    160661-plasmid-map-sequence-id-338890

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  9. HA-Clover-HIF-1alpha Wildtype

    Plasmid
    #163365
    Purpose
    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163365-plasmid-map-sequence-id-322246

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  10. MSCV H-Ras V12 IRES GFP

    Plasmid
    #18780
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 (HRAS Human)
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Mutation
    V12
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18780-plasmid-map-sequence-id-159508

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  12. TBG-Vector

    Plasmid
    #109396
    Purpose
    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver
    Depositor
    Sidi Chen
    Article
    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109396-plasmid-map-sequence-id-222776

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  13. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175508
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175508-plasmid-map-sequence-id-345097

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  16. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    61719-plasmid-map-sequence-id-175330

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  18. TFORF2593

    Plasmid
    #142336
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BHLHE40 (BHLHE40 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142336-plasmid-map-sequence-id-397187

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  31. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138566-plasmid-map-sequence-id-274798

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  35. pcDNA3-cdk5GFP

    Plasmid
    #1346
    Depositor
    Li-Huei Tsai
    Insert
    CDK5 (CDK5 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    1346-plasmid-map-sequence-id-156236

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  39. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  47. mCherry-Alpha-5-Integrin-12

    Plasmid
    #54970
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Alpha-5 Integrin (ITGA5 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    54970-plasmid-map-sequence-id-218701

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  48. mEmerald-LASP1-N-10

    Plasmid
    #54142
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    NM_006148 (LASP1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    A209V
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 11201 - 11250 of 76418 results