75,820 results

1. pSLQ1371-sgSTAT3-1

   Plasmid

   #121425

   Purpose

   sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgSTAT3-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX304 CMV FKBP-V5-sTurboID (N)

   Plasmid

   #153002

   Purpose

   Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-Soma-jGCaMP8m

   Plasmid

   #169257

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-L4R1-hEF1a

   Plasmid

   #139523

   Purpose

   Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   hEF1a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TOM20*-SBP-GFP

   Plasmid

   #120173

   Purpose

   Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
   

   Use

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Mutation

   Truncated TOM20: 1-30 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX301

   Plasmid

   #25895

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. V5-LOV-Turbo1-G3BP1_pTRE-G418

   Plasmid

   #202015

   Purpose

   expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-LOV-Turbo1-G3BP1
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-Tight

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL-p27UTR

    Plasmid

    #20881

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p27 3'UTR (Cdkn1b Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC035 - dLwCas13a-msfGFP

    Plasmid

    #91925

    Purpose

    Expresses catalytically inactive LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS-3xHA and NLS-msfGFP

    Expression

    Mammalian

    Mutation

    R488A and R1060A

    Promoter

    EF-1a

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3/Myc-DNMT1

    Plasmid

    #36939

    Purpose

    Mammalian expression of human DNMT1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Sema4D-Flag

    Plasmid

    #190646

    Purpose

    Expresses Mouse Sema4D with C Terminal Flag Tag

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-sp-myc-mSema4D

    Plasmid

    #190645

    Purpose

    Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_SybBC10

    Plasmid

    #240997

    Purpose

    Expression of sybody SLC66A1 in mammalian cells

    Depositor

    Simon Newstead

    Insert

    Syb-BC10-Myc-His6
    

    Use

    Tags

    Myc and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. NanoBRET Assay Vector, MYC

    Plasmid

    #237142

    Purpose

    Express Myc-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    Myc (MYC Human)
    

    Use

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. LgBiT-PIK3CA Fusion Vector

    Plasmid

    #237092

    Purpose

    Express LgBiT-PIK3CA Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. SmBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid

    #237079

    Purpose

    Express SmBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Nluc-YAP1_TEAD4-HaloTag Bidirectional(Balanced) Vector

    Plasmid

    #237003

    Purpose

    Express NLuc-YAP1_TEAD4-HaloTag(R) Fusion Proteins in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    YAP1 (YAP1 Human)
    TEAD4 (TEAD4 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

    Plasmid

    #231372

    Purpose

    Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

    Depositor

    David Bartel

    Article

    Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

    Insert

    Human Argonaute2 (AGO2 Synthetic, Human)
    

    Use

    Tags

    3xFLAG-SUMO_Eu1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGMC00035 (aka pMG0784)

    Plasmid

    #210768

    Purpose

    Lentiviral vector to express human ADRM1

    Depositor

    Raj Chari

    Article

    Osei-Amponsa et al Mol Cell. 2024 Feb 1;84(3):522-537.e8. d

    Insert

    ADRM1 (ADRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEBTetD-SNAP-Cep27

    Plasmid

    #136809

    Purpose

    Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep27 (HAUS2 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3-STAT3-Y705F

    Plasmid

    #74434

    Purpose

    Expression of STAT3-Y705F

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Mutations E16K, Y705F

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 Mfn2-HA

    Plasmid

    #139192

    Purpose

    Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PGK mScarlet-Synaptophysin

    Plasmid

    #206145

    Purpose

    PGK mScarlet-Synaptophysin

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    synaptophysin (Syp Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGG-NFIX1

    Plasmid

    #112702

    Purpose

    Expression of NFIX1 protein and GFP

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I X1 (Nfix Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Use

    Tags

    TurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    13X repeated p21-p53 response element

    Available since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid

    #58993

    Purpose

    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EcoRV site in MCS converted to BamHI site

    Promoter

    CMV enhancer-chicken beta actin

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPB-cT3G-cERP2-CEBPD

    Plasmid

    #192897

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of CEBPD

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    CEBPD (CEBPD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCherry-ER-3

    Plasmid

    #55041

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TFORF1448

    Plasmid

    #142796

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR5A2 (NR5A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-hNurr1-NE

    Plasmid

    #102363

    Purpose

    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    

    Use

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmVen-DHX9

    Plasmid

    #211409

    Purpose

    mammalian expression of human DHX9 N-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ERroGFP_iE_pCDNA3

    Plasmid

    #47954

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Ub-R-GFP

    Plasmid

    #11939

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid

    #172910

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2-ST
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti6-MK1-EHMT2-V5

    Plasmid

    #31113

    Depositor

    Bernard Futscher

    Insert

    euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Stop codon mutated to Ala

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pC0040-LwaCas13a crRNA backbone

    Plasmid

    #103851

    Purpose

    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-LwaCas13aDR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3.3_d2eGFP

    Plasmid

    #26821

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-d2eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    destabilized (PEST sequence) GFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. TFORF2987

    Plasmid

    #144463

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RORA (RORA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. Lamp1_mStayGold

    Plasmid

    #221902

    Purpose

    Expresses mStayGold fused to Lamp1

    Depositor

    Janelia Integrative Imaging

    Insert

    Lamp1_mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge