Skip to main content
Addgene
Showing: 5001 - 5050 of 37779 results
  1. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid
    #177316
    Purpose
    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.
    Depositor
    Insert
    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pAM4891 (pCV0003)

    Plasmid
    #120080
    Purpose
    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.
    Depositor
    Insert
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    Use
    Other
    Tags
    Expression
    Mutation
    mobA Y25F
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pMM627

    Plasmid
    #112532
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR
    Depositor
    Inserts
    HrtR
    ChuA
    luxCDABE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23107, PL(HrtO), and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D64V
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. gRNA NIA TLS2

    Plasmid
    #196981
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.
    Depositor
    Insert
    AtNIA1 gRNAs fused to TLS2 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. gRNA NIA TLS1

    Plasmid
    #196980
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.
    Depositor
    Insert
    AtNIA1 gRNAs fused to TLS1 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. Lenti-AsCpf1-Blast

    Plasmid
    #84750
    Purpose
    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Insert
    AsCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. psPAX2-D64V-NC-PP7

    Plasmid
    #122945
    Purpose
    PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles
    Depositor
    Insert
    NC-PCP (PP7 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D64V
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Insert
    GEM-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
  15. pMBEC2

    Plasmid
    #187596
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance
    Depositor
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 5001 - 5050 of 37779 results