8,687 results

1. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMo130-TelR

   Plasmid

   #50799

   Purpose

   Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

   Depositor

   Kim Lee Chua

   Article

   Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

   Insert

   tellurite-resistance cassette
   

   Use

   Suicide vector

   Tags

   Expression

   Mutation

   Promoter

   PCs12

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-NORAD

   Plasmid

   #120383

   Purpose

   Expression vector for lncRNA NORAD

   Depositor

   Igor Ulitsky

   Article

   Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

   Insert

   NORAD (NORAD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC016 - LwCas13a guide expression backbone with U6 promoter

   Plasmid

   #91906

   Purpose

   Backbone for cloning LwCas13a guides under U6 promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Has Service

   Cloning Grade DNA

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MigR1-AE

   Plasmid

   #12431

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

   Insert

   AML1-ETO fusion (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 gfaABC1D-tdTomato

   Plasmid

   #44332

   Depositor

   Baljit Khakh

   Article

   Shigetomi et al J Gen Physiol. 2013 Apr 15.

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV; Adeno-associated virus ()

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-PE6f

   Plasmid

   #207856

   Purpose

   Mammalian expression of PE6f prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6f
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

   Plasmid

   #86674

   Purpose

   Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   GFP-FLAG-cGAS E225A/D227A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lentiviral-FOP-dGFP-reporter

    Plasmid

    #14885

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    mutant LEF-1/TCF-responsive promoter (Ctnnb1 )
    

    Use

    Lentiviral

    Tags

    d2 eGFP

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Promoter

    Available since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLSSmOrange-C1

    Plasmid

    #37131

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-VHL-pRc/CMV

    Plasmid

    #19999

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-HIF1 alpha

    Plasmid

    #21101

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUCM-CLYBL-hNIL

    Plasmid

    #105841

    Purpose

    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF1a_mCherry_P2A_Hygro

    Plasmid

    #135003

    Purpose

    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-HRP-C

    Plasmid

    #40163

    Depositor

    Paul Ortiz de Montellano

    Article

    Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

    Insert

    Horseradish peroxidase
    

    Use

    Coli cloning vector

    Tags

    Six histidine

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. BE4-Gam

    Plasmid

    #100806

    Purpose

    Expresses BE4-Gam in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

    Insert

    BE4-Gam
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid

    #100843

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a-GlnRS-His

    Plasmid

    #124108

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutaminyl-tRNA ligase (glnS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mEmerald-Mito-7

    Plasmid

    #54160

    Purpose

    Localization: Mitochondria, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ZsGreen1-N1

    Plasmid

    #54702

    Purpose

    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    ZsGreen1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDsRed-Max-N1

    Plasmid

    #21718

    Depositor

    Benjamin Glick

    Article

    Strack et al Nat Methods. 2008 Nov . 5(11):955-7.

    Insert

    DsRed-Max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Pet28a_Human_H4

    Plasmid

    #42633

    Depositor

    Joe Landry

    Insert

    Human H4 (H4C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX304 Luciferase-V5 blast

    Plasmid

    #98580

    Purpose

    Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pGL3 1kb prom. CD274

    Plasmid

    #107002

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-ccdB-3xFLAG-V5

    Plasmid

    #87063

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-Bx

    Plasmid

    #51552

    Purpose

    Expresses Bxb1 integrase in mammalian cells.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    Bxb1 integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SIRT1 Flag

    Plasmid

    #13812

    Purpose

    Mammalian expression of human SIRT1 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    N-terminus truncated

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge