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Showing: 4161 - 4200 of 158741 results

  6. PB-CMV-MCS-Puro-EGFP

    Plasmid
    #219795
    Purpose
    Mammalian expression plasmids using piggyBac Transposon System
    Depositor
    Keiichi Nakayama
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219795-plasmid-map-sequence-id-441250

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  8. TFORF0058

    Plasmid
    #143007
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAF (MAF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143007-plasmid-map-sequence-id-408960

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  15. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-237587

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  16. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  20. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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  29. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    172481-plasmid-map-sequence-id-345344

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  32. TFORF0884

    Plasmid
    #142702
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOXB4 (HOXB4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142702-plasmid-map-sequence-id-397781

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  34. AbVec2.0-IGHG3

    Plasmid
    #99577
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99577-plasmid-map-sequence-id-192592

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  36. pRL528

    Plasmid
    #58495
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al Methods Enzymol. 1988;167:747-54.
    Insert
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58495-plasmid-map-sequence-id-107317

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Showing: 4161 - 4200 of 158741 results