4,132 results

1. pgRNA-modified

   Plasmid

   #84477

   Purpose

   guide RNA expression lentiviral construct

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   guide RNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag-HA-USP14

   Plasmid

   #22569

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP14 (USP14 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CAV1-mEGFP

   Plasmid

   #27704

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXs-puro-RFP-ATG9A

   Plasmid

   #60609

   Purpose

   Expresses RFP-hATG9A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 9A (PPARG Human)
   

   Use

   Retroviral

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLOVE-Klf4

   Plasmid

   #15950

   Depositor

   Miguel Ramalho-Santos

   Article

   Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

   Insert

   Kruppel-like factor 4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   The recombination reaction required to clone &quo…

   Promoter

   Available since

   Nov. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFPN1-human Dynamin 1aa K44A

   Plasmid

   #22197

   Depositor

   Pietro De Camilli

   Article

   Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

   Insert

   human Dynamin 1aa K44A (DNM1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DsRed2-pBAD

   Plasmid

   #54608

   Purpose

   Localization: Protein Expression Vector , Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   6xHis and DsRed2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCS6

   Plasmid

   #55752

   Purpose

   Produces T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   T7 RNA Polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

   Plasmid

   #141367

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-FRB

    Plasmid

    #25919

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmTurquoise2-ER

    Plasmid

    #36204

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    ER-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDIRE

    Plasmid

    #26745

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    iCre expression cassette
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA Flag-RARalpha

    Plasmid

    #35555

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LRP6-pCS2

    Plasmid

    #27242

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid

    #22028

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid

    #22029

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant).

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA-mTmG

    Plasmid

    #26123

    Depositor

    Liqun Luo

    Insert

    Precursor mT/mG
    

    Use

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC-GAL4

    Plasmid

    #24344

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RelB cFlag pcDNA3

    Plasmid

    #20017

    Depositor

    Stephen Smale

    Insert

    RelB (Relb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Flag-HA-USP13

    Plasmid

    #22568

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP13 (USP13 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-gRNA

    Plasmid

    #46759

    Purpose

    gRNA cloning vector for in vitro transcription of target gRNA

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    gRNA core
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. dsRed-cent2

    Plasmid

    #29523

    Depositor

    Joseph Gleeson

    Article

    Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

    Insert

    Cent2 (CETN2 Human)
    

    Use

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPAGFP-N1

    Plasmid

    #11909

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    pPAGFP-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge