Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

173,847 results

Showing: 5301 - 5350 of 173847 results

  23. pRS159

    Plasmid
    #122378
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122378-plasmid-map-sequence-id-241243

    Enlarge

  24. ADRA1A-Tango

    Plasmid
    #66213
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA1A (ADRA1A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66213-plasmid-map-sequence-id-369270

    Enlarge

  28. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168125-plasmid-map-sequence-id-332869

    Enlarge

  37. pcDNA3-Par6-ExRai-CKAR2

    Plasmid
    #236101
    Purpose
    Subcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Par6C-ExRai-CKAR2 (PARD6A Human, Synthetic)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236101-plasmid-map-sequence-id-473796

    Enlarge

  38. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

    Enlarge

  41. pSBbi-Pur

    Plasmid
    #60523
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60523-plasmid-map-sequence-id-305758

    Enlarge

  43. pBabepuro-OsTIR1(F74G)-9xMyc

    Plasmid
    #250508
    Purpose
    Retroviral vector for stable expression of OsTIR1(F74G)-9xMyc in mammalian cell lines
    Depositor
    Kai Ge
    Insert
    TIR1(F74G)
    Use
    Retroviral
    Tags
    9xMyc
    Expression
    Mammalian
    Available Since
    Jan. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    250508-plasmid-map-sequence-id-497809

    Enlarge

  45. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Promoter
    CBh; U6
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

    Enlarge

  49. pSBbi-TIR1-tTA/Pur

    Plasmid
    #171683
    Purpose
    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)
    Depositor
    Randy Poon
    Article
    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.
    Inserts
    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    Expression
    Mammalian
    Promoter
    EF-1a promoter and RPBSA promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171683-plasmid-map-sequence-id-338224

    Enlarge

  50. mEmerald-Vinculin-23

    Plasmid
    #54302
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5301 - 5350 of 173847 results