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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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163,606 results

Showing: 5451 - 5500 of 163606 results

  5. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR1 (IFNGR1 Human)
    Use
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192786-plasmid-map-sequence-id-379664

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  13. mEmerald-CLIP170-N-18

    Plasmid
    #54044
    Purpose
    Localization: Microtubule Binding , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CLIP170 (CLIP1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54044-plasmid-map-sequence-id-139284

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  17. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid
    #122846
    Purpose
    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    human FASN/ human fatty acid synthase (FASN Human)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122846-plasmid-map-sequence-id-238539

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  18. pLenti6MFV SLC10A1

    Plasmid
    #194130
    Purpose
    Expresses NTCP (SLC10A1) protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC10A1 (SLC10A1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194130-plasmid-map-sequence-id-380754

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  23. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid
    #111727
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q54 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111727-plasmid-map-sequence-id-272590

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  31. AAV-SYN-mBaoJin-oPRE

    Plasmid
    #218683
    Purpose
    AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218683-plasmid-map-sequence-id-447610

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  34. pcDNA3.1-StrepII

    Plasmid
    #208617
    Purpose
    Empty vector for mammalian expression, insert a Strep II tag (with ATG) using XbaI and XhoI site of pcDNA3.1 (-), both site retained
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Strep II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208617-plasmid-map-sequence-id-418313

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  41. ADRA2A-Tango

    Plasmid
    #66216
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2A (ADRA2A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66216-plasmid-map-sequence-id-417865

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  42. STING-V2

    Plasmid
    #124264
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus2
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124264-plasmid-map-sequence-id-241461

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Showing: 5451 - 5500 of 163606 results