We narrowed to 7,223 results for: ALP

1. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

   Plasmid

   #26567

   Depositor

   Diane Lipscombe

   Article

   Lin et al Neuron. 1997 Jan . 18(1):153-66.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons a10, 31a, 37b, and 46. Deletion…

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pJT25_GalL_BE3

   Plasmid

   #143736

   Purpose

   Expresses BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

   Plasmid

   #26569

   Depositor

   Diane Lipscombe

   Article

   Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

   Insert

   calcium channel alpha-1B subunit (Cacna1b Rat)
   

   Use

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Deletion of exons a10, 18a, and 24a. Contains …

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. AV4-EF1-NMNAT2

   Plasmid

   #124970

   Purpose

   Express NMNAT2

   Depositor

   Kazuhiro Oka

   Article

   Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

   Insert

   NMNAT2, GFP (NMNAT2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1-alpha

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pROF402

   Plasmid

   #155334

   Purpose

   Binary vector for expression of FLuc controlled by the blue light-repressible system 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
   

   Use

   Luciferase and Synthetic Biology; Eukaryotic expr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pROF441

   Plasmid

   #155336

   Purpose

   Binary vector for expression of a gRNA targeting AtAP1 promoter 

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5 HA-GST-Presc-mAkt1 (wt)

   Plasmid

   #48805

   Purpose

   expresses wt Akt1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA-GST-Presc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. SARS_COV2_orf9c_HA_BASU

   Plasmid

   #153991

   Purpose

   Express Orf9c gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf9c
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b-Casp8 delta DED C360A

   Plasmid

   #11828

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Minus Death Defecter Domain (aa 1-216), C360A

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

10. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

    Plasmid

    #26571

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 31a Contains e…

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEBB COMMD9 Flag

    Plasmid

    #74901

    Purpose

    Express COMMD9 in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD9 (COMMD9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEBB COMMD8 Flag

    Plasmid

    #74900

    Purpose

    Express COMMD8 in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD8 (COMMD8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGG-TurboID-GFP-PTS1

    Plasmid

    #209406

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-PTS1
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

    Plasmid

    #78348

    Purpose

    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-KRAB
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBSFI-myr-akt-S473A

    Plasmid

    #49190

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    S473A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psicheck-2-Casp8

    Plasmid

    #48172

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of CASP8 (CASP8 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pDB.His.MBP.3C NLRP1 CARD WT

    Plasmid

    #164008

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

    Plasmid

    #44195

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

    Expression

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

    Plasmid

    #148839

    Purpose

    Mammalian Expression of DmBam_13-36

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_13-36 (bam Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJ128

    Plasmid

    #19962

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEBB COMMD4 Flag

    Plasmid

    #74896

    Purpose

    Express COMMD4 in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD4 (COMMD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBSFI-myr-akt-T308A

    Plasmid

    #49189

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MBP-GTSE1-His6

    Plasmid

    #69109

    Purpose

    For bacterial expression of recombinant human GTSE1 tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    G2- and S-phase Expressed 1 (GTSE1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BACE2_pCSdest

    Plasmid

    #53915

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    BACE2 (BACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

30. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26570

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pEBB COMMD7 Flag

    Plasmid

    #74899

    Purpose

    Express COMMD7 in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD7 (COMMD7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLVE-FH-IN

    Plasmid

    #70071

    Purpose

    2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV-CAGGS-mNAGA

    Plasmid

    #196448

    Purpose

    Expresses modified α-N-acetylgalactosaminidase in mammalian cells

    Depositor

    Yuichiro Miyaoka

    Article

    Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

    Insert

    modified alpha-N-acetylgalactosaminidase (NAGA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCIneoLuc-PP1A

    Plasmid

    #128348

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Clover-Tubulin-C-18

    Plasmid

    #56374

    Purpose

    Localization: Microtubules, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HSF1 KO

    Plasmid

    #200207

    Purpose

    gRNA for HSF1 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF1 KO (Hsf1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-REPORT(EF1a)-TTN

    Plasmid

    #172327

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMpGWB321

    Plasmid

    #68649

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEBB COMMD6 Flag

    Plasmid

    #74898

    Purpose

    Express COMMD6 in mammalian cells

    Depositor

    Ezra Burstein, Colin Duckett

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    COMMD6 (COMMD6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid

    #101347

    Purpose

    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta Tat

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-sCAG-GluClβ.CreON

    Plasmid

    #196996

    Purpose

    Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

    Depositor

    David Bennett

    Article

    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

    Insert

    GluClβ -YFP
    

    Use

    AAV and Cre/Lox

    Tags

    YFP

    Expression

    Mutation

    Promoter

    short CAG

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. ABCA1 promoter luciferase reporter (P1 fragment)

    Plasmid

    #86443

    Purpose

    Human ABCA1 promoter luciferase reporter (P1 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pFETCh_GATAD2A

    Plasmid

    #86279

    Purpose

    Donor vector for 3' FLAG tag of human GATAD2A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    GATAD2A homology arms (GATAD2A Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pBS-AMV-α1L9'S-nAchR

    Plasmid

    #20684

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    nicotinic acetylcholine receptors (Chrna1 Mouse)
    

    Use

    Tags

    AMV and pA 50

    Expression

    Bacterial

    Mutation

    Leucine at 9' position in M2 region changed …

    Promoter

    Available since

    April 3, 2009

    Availability

    Academic Institutions and Nonprofits only

49. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

50. pDONR221 NLRP1 P1214R

    Plasmid

    #166822

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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