8,883 results

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ArcLight-Q239

Plasmid

#36856

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

simian CMV

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Tags

6xHis and FusionRed

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway entry vector

Expression

Mammalian

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-UtrCH

Plasmid

#26740

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

mCherry

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-NLuc

Plasmid

#31181

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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paGFP-Omp25

Plasmid

#69598

Purpose

expresses GFP-labeled mitochondrial outer membrane protein 25

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

paGFP-Omp25 (Synj2bp Rat)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

photoactivatable GFP fused to rat Omp25 C-terminus

Promoter

CMV

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCGN-SRF

Plasmid

#11977

Purpose

For mammalian expression of human SRF with an HA tag

Depositor

Ron Prywes

Article

Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Insert

SRF (SRF Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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RCASBP-Y DV

Plasmid

#11478

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Available Since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV PIG (Puro IRES GFP)

Plasmid

#18751

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJ3M-Mst1 K59R

Plasmid

#12204

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst1 (STK4, MST1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive: K59R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin E

Plasmid

#8963

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin E (CCNE1 Human)

Tags

GPI linker

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 IKKE K38A flag

Plasmid

#26202

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon K38A (IKBKE Human)

Tags

Flag

Expression

Mammalian

Mutation

Catalytic Lysine changed to Alanine (K38A)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag TBK1

Plasmid

#20648

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-Peroxi

Plasmid

#36203

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Peroxisomal targeting sequence

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2-tdtomato

Plasmid

#18917

Purpose

Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-tdtomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdtomato

Promoter

CAG

Available Since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ALB-bio-His

Plasmid

#52176

Purpose

Expresses full-length Serum albumin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ALB (ALB Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 TDP-43 5F-L YFP

Plasmid

#84914

Purpose

Mammalian expresion of TDP-43 5F-L YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Mutation

F147L, F149L, F194L, F229L, F231L

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKAM-BFP-Puro

Plasmid

#101864

Purpose

Lentiviral empty backbone, dual BFP and puro markers

Depositor

Archibald Perkins

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

Plasmid

#32739

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (Tcf7l2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N-terminal deletion form (delta N) lacks amin…

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFast-FT-N1

Plasmid

#31910

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
GeNL/pcDNA3

Plasmid

#85200

Purpose

Green color variant of bright luminescent protein

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

Green enhanced Nano-lantern

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP C3-YAP2

Plasmid

#19055

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-Cdc42-wt

Plasmid

#12972

Depositor

Gary Bokoch

Insert

Cdc42 (CDC42 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLenti CMV/TO Puro empty (w175-1)

Plasmid

#17482

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-IF1

Plasmid

#124123

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 1 (infA Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMVInt

Plasmid

#18935

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESneo-FLAG/HA Ago1

Plasmid

#10820

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

Argonaute 1 (AGO1 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway cloning

Tags

mCherry and mVenus

Available Since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pfmh-hCry1

Plasmid

#25843

Depositor

Aziz Sancar

Insert

human Cryptochrome 1 (CRY1 Human)

Tags

flag, his, and myc

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
CMV-B-GECO1

Plasmid

#32448

Purpose

Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

CMV

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

Plasmid

#71720

Purpose

Expressing an mRuby-tagged TIR1 in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)

Tags

mRuby

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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