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162,300 results

Showing: 6251 - 6300 of 162300 results

  1. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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  3. pCAG-VSVG

    Plasmid
    #64084
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV glycoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64084-plasmid-map-sequence-id-107221

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  4. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  8. HypoxCR

    Plasmid
    #59946
    Purpose
    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
    Depositor
    Anne Le
    Article
    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p
    Inserts
    GFP-Pest
    mCherry-Geminin
    Use
    Lentiviral
    Tags
    FLAG and PEST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and min CMV
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    59946-plasmid-map-sequence-id-228094

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  11. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  15. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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  23. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  28. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  33. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  34. pCXLE-SOX2-17-p2a-KLF4

    Plasmid
    #193290
    Purpose
    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193290-plasmid-map-sequence-id-379665

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  39. pENTR_AtU6p_BsaI_gRNA

    Plasmid
    #225987
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225987-plasmid-map-sequence-id-448010

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  43. RBD-Fc

    Plasmid
    #164221
    Purpose
    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter
    Depositor
    Sriram Neelamegham
    Article
    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1
    Insert
    Spike RBD (S SARS-CoV)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    164221-plasmid-map-sequence-id-323637

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  47. iOn-CAG∞MCS

    Plasmid
    #154013
    Purpose
    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    MCS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154013-plasmid-map-sequence-id-307168

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  48. pX330_TOMM20 sgRNA / hSpCas9

    Plasmid
    #172836
    Purpose
    Mammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172836-plasmid-map-sequence-id-340903

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Showing: 6251 - 6300 of 162300 results