161,256 results
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pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-WT
Plasmid#25361DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115 and T7/lacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-200u
Plasmid#13555DepositorInsertFLIPPi G162A
UseTagsECFP and EYFPExpressionBacterialMutationG162A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3
Plasmid#170163PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-P2A-H2B-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 HRS-RFP
Plasmid#29685DepositorInserthepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKH3-human RSK1
Plasmid#13841DepositorInsertRSK1 (RPS6KA1 Human)
UseTags3x HAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
p50 cFlag pcDNA3
Plasmid#20018DepositorInsertNfkb1 (Nfkb1 Mouse)
UseTagsFlagExpressionMammalianMutationTruncated at the p105 ligation site. It codes for…PromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIVTRup
Plasmid#101362PurposeHighly efficient in vitro transcription. Empty backbone.DepositorInsert5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
UseFor cloning a gene for in vitro transcription pur…TagsExpressionMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE
Plasmid#140086PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-Puro-T2A-BFPExpressionMammalianMutationPromoterU6Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK265 (RAD21-mAC Hygro)
Plasmid#140539PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEH371 CD63 HA
Plasmid#168220PurposeMammalian expression of tetraspanin CD63 fused to a C-terminal HA tagDepositorInsertCD63 (CD63 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP2/pRK5
Plasmid#27973DepositorInsertCIAP2 (BIRC3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVie-IRES-BlastR
Plasmid#119863Purpose3rd generation lentiviral expression under a CMVie promoter with Blasticidin selectionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPa-NRX
Plasmid#73143PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.DepositorInsertsHRPa-NRX
UseTags2x HA tag and FlagExpressionMammalianMutationPromoterCAGAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorInsertsHRPb-NLG (Nlgn1 Mouse, Synthetic)
UseTagsV5 tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBE-Luc
Plasmid#16527DepositorInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40
Plasmid#110154PurposeExpression of human BHLHE40 in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIRT4 Flag
Plasmid#13815PurposeMammalian expression of human SIRT4 with flag tagDepositorInsertSIRT4 (SIRT4 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
UseTagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-MARK2-eGFP
Plasmid#66706Purposemammalian expression vector for MARK2 with a C-terminal eGFPDepositorInsertMARK2 (MARK2 Human)
UseTagseGFPExpressionMammalianMutationPromoterchicken beta actinAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA
Plasmid#163024PurposeExpression of CRISPRi with U6-gRNA (Bsa1 sites)DepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 polyA, and myc NLSExpressionMammalianMutationPromoterhEF-1aAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP WT
Plasmid#123097PurposeExpresses 3xFLAG-GABARAP wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
UseTagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only
