We narrowed to 8,360 results for: construct

1. pUC19/Tp-mChe

   Plasmid

   #183165

   Purpose

   Construct for transient expression of tonoplast-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Tp-mCherry
   

   Use

   Tags

   28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. OPN3-DuET

   Plasmid

   #213357

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   OPN3 (OPN3 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Flag-Cortactin

   Plasmid

   #74476

   Purpose

   This plasmid was constructed to study cortactin interactions

   Depositor

   J Thomas Parsons

   Article

   Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

   Insert

   Cortactin (Cttn Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. cyt-GafD-mTurboID-V5

   Plasmid

   #184640

   Purpose

   Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   cyt-GlycoID-V5
   

   Use

   Tags

   Nuclear export sequence and V5 tag

   Expression

   Mammalian

   Mutation

   GafD -> expressed aa22-178 ; BirA* -> expre…

   Promoter

   CMV

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR-CyOFP1

   Plasmid

   #228604

   Purpose

   Mammalian expression of CyOFP1, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   CyOFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P2RY11-DuET

   Plasmid

   #213364

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   P2RY11 (P2RY11 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Biomolecular FeedBack (FB)

   Plasmid

   #109391

   Purpose

   This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pJ23100mut-dCas9-T- phtpG1-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

   Plasmid

   #194221

   Purpose

   Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-mEGFP-PKCa-C1

   Plasmid

   #112264

   Purpose

   ITRACKalpha Donor Construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   protein kinase C alpha (PRKCA Bovine)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   A11V (please see depositor comments below)

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Use

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUB6-CDC34-HA (pRKB261)

    Plasmid

    #99145

    Purpose

    Mammalian expression construct to express HA-tagged CDC34

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    CDC34 (CDC34 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NTSR1-DuET

    Plasmid

    #213356

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NTSR1 (NTSR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Comments

    Promoter

    CaMKII

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-human CSF-1Rwt-IRES-GFP

    Plasmid

    #115523

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    human CSF-1 receptor (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid

    #86194

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta Globin (HBB, Hbb Mouse, Human)
    

    Use

    Tet on

    Tags

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Tet on

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OPRD1-DuET

    Plasmid

    #213358

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRD1 (OPRD1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

    Plasmid

    #222992

    Purpose

    Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SSTR2-DuET

    Plasmid

    #213377

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR2 (SSTR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Akt-Scramble

    Plasmid

    #175014

    Purpose

    Negative-control construct for Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-Scramble
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG_EGFP-RandE3

    Plasmid

    #79870

    Purpose

    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SiC-V2-Cas9G7

    Plasmid

    #133043

    Purpose

    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    Tet repressor
    

    Use

    Lentiviral

    Tags

    Cerulean

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBMN-AS-YY1

    Plasmid

    #154943

    Purpose

    Knocking down of human YY1 through shRNA and amiRNA targeting YY1

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    shRNA and amiRNA against YY1 (YY1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid

    #102885

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBMN-2xFKBP-mEGFP-NDP52

    Plasmid

    #110465

    Purpose

    retrovirus construct for stable expressing mEGFP tagged NDP52

    Depositor

    Richard Youle

    Article

    Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral; Retrovirus

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pN006

    Plasmid

    #188470

    Purpose

    Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    AsCas12a
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pN256

    Plasmid

    #188472

    Purpose

    scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    eGFP-WPRE-DAISY-4T
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ErbB-3-pGL3

    Plasmid

    #60899

    Purpose

    human ErbB-3 promoter reporter construct

    Depositor

    Frederick Domann

    Article

    Zhu et al Breast Cancer Res Treat. 2002 Jan;71(1):

    Insert

    ErbB-3 promoter (ERBB3 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ACE2(-252)-luc

    Plasmid

    #31111

    Purpose

    Luciferase reporter construct for human ACE2 promoter

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    ACE2 promoter (ACE2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    promoter region -252 to +103

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

40. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. RXFP1-DuET

    Plasmid

    #213373

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    RXFP1 (RXFP1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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42. pEB2_mScarletI_mVenusNB-MrkHR113A

    Plasmid

    #182292

    Purpose

    expresses c-di-GMP mutant biosensor in E. coli

    Depositor

    Howard Berg

    Article

    Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

    Insert

    mVenusNB-MrkHR113A
    

    Use

    Tags

    mVenusNB internal fusion to MrkH with a R113A poi…

    Expression

    Bacterial

    Mutation

    changed arginine 113 to alanine, MrkH numbering …

    Promoter

    proC

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. TA1-DuET

    Plasmid

    #213382

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    TA1 (TAAR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. YF-Cdc42(CA)

    Plasmid

    #20152

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP-Cdc42(CA) (CDC42 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cdc42H was inserted into the multiple cloning sit…

    Promoter

    Available since

    March 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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46. pU6-Universal.Sth.dCas9

    Plasmid

    #171088

    Purpose

    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    

    Use

    Toxoplasma gondii expression

    Tags

    Expression

    Mutation

    Note: A S245F mutation is present but did not app…

    Promoter

    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Ikuko Hara-Nishimura, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

    Plasmid

    #78509

    Purpose

    Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD45R0Ex (PTPRC Human)
    

    Use

    Tags

    His10 and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. pOPS0263

    Plasmid

    #133228

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pOPS0262

    Plasmid

    #133227

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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