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8,637 results

Showing: 6801 - 6850 of 8637 results

  3. pK7WGF2::hCas9

    Plasmid
    #46965
    Purpose
    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
    Depositor
    Sophien Kamoun
    Article
    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.
    Insert
    hCas9
    Use
    CRISPR; Plant expression
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    35S
    Available since
    Aug. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    46965-plasmid-map-sequence-id-66887

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  6. pDisplay-HRP-KDEL

    Plasmid
    #85582
    Purpose
    Expresses HRP-KDEL in mammalian cells
    Depositor
    Alice Ting
    Insert
    HRP-KDEL
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    85582-plasmid-map-sequence-id-165896

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  8. pAAV-CaMKIIa-eNpHR 3.0-EYFP

    Plasmid
    #26971
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV1 and AAV9
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Trafficking Signal (TS) ER Export Signal
    Promoter
    CaMKIIa
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26971-plasmid-map-sequence-id-196840

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  14. p52 cFlag pcDNA3

    Plasmid
    #20019
    Depositor
    Stephen Smale
    Insert
    Nfkb2 (Nfkb2 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p100 ligation site. It codes for…
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  22. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  23. pdCas9::BFP-humanized

    Plasmid
    #44247
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 fused to BFP
    Use
    CRISPR and Retroviral
    Tags
    2xNLS & tagBFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44247-plasmid-map-sequence-id-60248

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  25. pUltra-Chili

    Plasmid
    #48687
    Purpose
    3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    48687-plasmid-map-sequence-id-70485

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  26. pRK5-myc-Cdc42-T17N

    Plasmid
    #12973
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T17N Dominant negative
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12973-plasmid-map-sequence-id-134936

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  34. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

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  41. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Kevin McGowan
    Insert
    Sec61 (SEC61B Human)
    Use
    Tags
    HaloTag7
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    123285-plasmid-map-sequence-id-242690

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  45. pmECFP-1

    Plasmid
    #38772
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Tags
    mECFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    38772-plasmid-map-sequence-id-48799

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  46. pCMVHA-E2F5

    Plasmid
    #24213
    Purpose
    Mammalian expression of human E2F5 with HA tag
    Depositor
    Kristian Helin
    Insert
    E2F5 (E2F5 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24213-plasmid-map-sequence-id-245679

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  47. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103849-plasmid-map-sequence-id-199152

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Showing: 6801 - 6850 of 8637 results