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8,963 results

Showing: 6801 - 6850 of 8963 results

  2. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87429-plasmid-map-sequence-id-174170

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  18. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #84445
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84445-plasmid-map-sequence-id-177041

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  21. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  24. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet

    Plasmid
    #137161
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Foff 2.0-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available Since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137161-plasmid-map-sequence-id-272782

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  33. pRK5-myc-Cdc42-Q61L

    Plasmid
    #12974
    Depositor
    Gary Bokoch
    Insert
    Cdc42 constitutively active (CDC42 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Available Since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    12974-plasmid-map-sequence-id-132746

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  38. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Expression
    Mammalian
    Promoter
    beta-actin
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

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  43. pAL149

    Plasmid
    #22275
    Purpose
    Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane
    Depositor
    Christopher Voigt
    Article
    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.
    Insert
    PhyB(1-908) (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    mCherry-Kras4BCT (prenylation sequence)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    22275-plasmid-map-sequence-id-207638

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  44. p50 cFlag pcDNA3

    Plasmid
    #20018
    Depositor
    Stephen Smale
    Insert
    Nfkb1 (Nfkb1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p105 ligation site. It codes for…
    Available Since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20018-plasmid-map-sequence-id-169435

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  49. mNeptune2-N1

    Plasmid
    #54837
    Purpose
    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54837-plasmid-map-sequence-id-162708

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  50. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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Showing: 6801 - 6850 of 8963 results