57,607 results
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pCDNA3-PAX3-FOXO1/FKHR
Plasmid#115526PurposeMammalian expression constructDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSNK1A1
Plasmid#23355DepositorInsertCSNK1A1 (CSNK1A1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorInsertTTF1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hciap2mut
Plasmid#8339DepositorInsertciap2mut (BIRC3 Human)
UseTagsmycExpressionMammalianMutationH574APromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A8
Plasmid#132233PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A8 (SLC7A8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRM14
Plasmid#163693Purposehsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarletDepositorUseUnspecified; Zebrafish expressionTagsmNeonGreen and mScarletExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-ErbB3
Plasmid#197362PurposeEncoding human ErbB3.DepositorInsertERBB3 (ERBB3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-Sec23B
Plasmid#66598PurposeExpresses Sec23B as a fusion with mGFPDepositorInsertSec23B (SEC23B Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Human Parkin (1-465)
Plasmid#110758PurposeBacterial expression of human ParkinDepositorInsertPRKN (PRKN Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
UseTagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p4054 HA-E6AP isoform II
Plasmid#8658DepositorInsertE6-AP isoform II (UBE3A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) SDC3
Plasmid#107501PurposeExpresses SDC3, puromycin resistantDepositorInsertSDC3 (codon optimized) (SDC3 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1
Plasmid#159576PurposeBaculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1DepositorInsertHis6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
UseTagsHis6ExpressionInsectMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt3
Plasmid#35909DepositorInsertWnt3 (WNT3 Human)
UseTagsExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human ERBIN
Plasmid#40214DepositorInsertERBIN (ERBIN Human)
UseTagsmycExpressionMammalianMutationL143S, S274L and P338L mutation in ERBIN compared…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_zeo_sgNT135
Plasmid#183181PurposepLentiCRISPR that is selectable with zeocin with a non-targeting sgRNADepositorInsertNT135
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5xUAS-Flag-NPM1c
Plasmid#131823Purpose4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transductionDepositorInsertNPM1 (NPM1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVL1392 TMEM97
Plasmid#157748PurposeBaculovirus transfer vectors to create a virus expressing human TMEM97DepositorInsertTMEM97 (TMEM97 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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TFORF1330
Plasmid#143950PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTFAP2C (TFAP2C Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PTK2
Plasmid#20610DepositorInsertPTK2 (PTK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBB-XIAPmut
Plasmid#11559DepositorInsertXIAPmut (XIAP Human)
UseTagsFlagExpressionMammalianMutationchanged his 467 to AlaninePromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF1317
Plasmid#143788PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRB1CC1 (RB1CC1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1c
Plasmid#131813PurposeExpresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationThis is the cytoplasmic mutant causing AML, where…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MRGPRD-DuET
Plasmid#213340PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMRGPRD (MRGPRD Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADAMTS5_pCSdest
Plasmid#53819DepositorInsertADAMTS5 (ADAMTS5 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
UseTagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArgininePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1
Plasmid#60540PurposeDonor vector for human MLK1DepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1106
Plasmid#143762PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEPAS1 (EPAS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TMEM192-FLAG
Plasmid#164977PurposeLentiviral vector for TMEM192 expression in mammalian cellsDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-hHDAC3
Plasmid#98591Purpose3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.DepositorInsertdCAS9-HDAC3 (HDAC3 S. pyogenes, Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iM
Plasmid#58973PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 WT in pET29b-Strep
Plasmid#48968PurposeExpresses Full length wild type MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF0113
Plasmid#141520PurposeLentiviral vector for overexpressing the SMAD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD3 (SMAD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0112
Plasmid#141519PurposeLentiviral vector for overexpressing the SMAD2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD2 (SMAD2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ Flag-RAP2A
Plasmid#128099PurposeExpresses RAP2A in mammalian cells in a G418-selectable plasmidDepositorInsertRAP2A (RAP2A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)
Plasmid#159539PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)DepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRM15
Plasmid#163694Purposehsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670DepositorUseZebrafish expressionTagsmScarlet and miRFP670ExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iG
Plasmid#58975PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
5E26 (PANK2)
Plasmid#79516PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPANK2 (PANK2 Human)
UseBaculovirus expressionTags6xHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
hCdh1 (1-125)
Plasmid#8508DepositorInserthCdh1 (1-125) (FZR1 Human)
UseRetroviralTagsHisExpressionMammalianMutationCdh1 N-terminus. Dominant Negative mutant.PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
SMC2 CRISPR
Plasmid#140649PurposeSMC2 tagging CRISPRDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBRII / CEP-Zeo / Hygro
Plasmid#16622DepositorInsertTGF beta type II receptor (TGFBR2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-Kinase
Plasmid#25071DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationContains only the kinase domain aa 1863-2218PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only
