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19,186 results

Showing: 7751 - 7800 of 19186 results

  2. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

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  5. p406ADH1

    Plasmid
    #15974
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15974-plasmid-map-sequence-id-8459

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  12. p4xSTAT6-Luc2P

    Plasmid
    #35554
    Depositor
    Axel Nohturfft
    Insert
    4xStat6 promoter
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    4xStat6
    Available since
    March 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    35554-plasmid-map-sequence-id-338744

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  13. RcCMV/Rb

    Plasmid
    #1763
    Depositor
    Bob Weinberg
    Insert
    human Rb (RB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only
    1763-plasmid-map-sequence-id-194429

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  14. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  16. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  17. pAAV-TRE-GCaMP6s

    Plasmid
    #135418
    Purpose
    Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.
    Depositor
    Rylan Larsen
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135418-plasmid-map-sequence-id-270731

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  28. mEmerald-CLIP170-N-18

    Plasmid
    #54044
    Purpose
    Localization: Microtubule Binding , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CLIP170 (CLIP1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54044-plasmid-map-sequence-id-139284

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  41. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

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  44. ADRB1-Tango

    Plasmid
    #66219
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB1 (ADRB1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66219-plasmid-map-sequence-id-456477

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  46. AIO-mCherry

    Plasmid
    #74120
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    74120-plasmid-map-sequence-id-275140

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  49. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162468-plasmid-map-sequence-id-350748

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Showing: 7751 - 7800 of 19186 results