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Showing: 7851 - 7900 of 157724 results

  3. ADRA2B-Tango

    Plasmid
    #66217
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2B (ADRA2B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. mEmerald-Calnexin-N-14

    Plasmid
    #54021
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. PSD-95-pTagRFP

    Plasmid
    #52671
    Purpose
    expression of TagRFP-tagged PSD-95
    Depositor
    Johannes Hell
    Insert
    PSD-95 (Dlg4 Rat)
    Use
    Tags
    tagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52671-plasmid-map-sequence-id-272433

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  6. 6CC8 (MBD3)

    Plasmid
    #119959
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD3 (MBD3 Human)
    Use
    Tags
    GSTtag, 6xHis and Thrombin
    Expression
    Bacterial
    Mutation
    MBD domain (contains residues 1-71)
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119959-plasmid-map-sequence-id-232658

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  11. PGAM5

    Plasmid
    #38976
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PGAM5 (PGAM5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pDAC627

    Plasmid
    #195240
    Purpose
    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195240-plasmid-map-sequence-id-457484

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  16. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

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  17. TFORF1108

    Plasmid
    #143254
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREBBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143254-plasmid-map-sequence-id-396151

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  19. TFORF0220

    Plasmid
    #142564
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP4 (FOXP4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142564-plasmid-map-sequence-id-391429

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  20. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

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  21. TFORF2692

    Plasmid
    #142358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142358-plasmid-map-sequence-id-397200

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  23. mCherry-Keratin14-N-18

    Plasmid
    #55066
    Purpose
    Localization: Cytokeratin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Keratin14 (KRT14 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. ADRA2C-Tango

    Plasmid
    #66218
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2C (ADRA2C Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid
    #113950
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113950-plasmid-map-sequence-id-224188

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  31. pcDNA3-TLR9-YFP

    Plasmid
    #13642
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 9 (TLR9 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13642-plasmid-map-sequence-id-392328

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  33. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    35700-plasmid-map-sequence-id-228311

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  42. pAAV-nEF-Con/Fon-ChRmine-oScarlet

    Plasmid
    #137159
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Fon-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137159-plasmid-map-sequence-id-272775

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  44. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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Showing: 7851 - 7900 of 157724 results