19,770 results

Showing: 7851 - 7900 of 19770 results

Results per page:

Sort results by:

pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 LIC cloning vector (6A)

Plasmid

#30124

Depositor

Scott Gradia

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

Plasmid

#162378

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-GSX2

Plasmid

#96964

Purpose

Dox-inducible expression of Gsx2 in mammalian cells

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Insert

GSX2 (Gsx2 Mouse)

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-neo-PP1-Alpha

Plasmid

#171267

Purpose

Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1.Gαi2-LgB91

Plasmid

#134358

Purpose

Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi2-LgB91 (GNAI2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
opto-RhoA-mCherry_pcDNA3.1

Plasmid

#164472

Purpose

Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoA

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-C1

Plasmid

#54631

Purpose

Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-SF2

Plasmid

#17990

Depositor

Tom Misteli

Article

Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

Insert

SF2 (SRSF1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains SF2 (aa1-210)

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6c:sgRNA#4

Plasmid

#64248

Purpose

expresses sgRNA under U6c promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-zeo largeTcDNA

Plasmid

#1779

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PAK4

Plasmid

#20460

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PAK4 (PAK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Article

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p006-GFP-pBAD

Plasmid

#108315

Purpose

GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

GFP under control of arabinose-inducible promoter pBAD

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p58sheLL

Plasmid

#37324

Depositor

John Schiller

Article

Kondo et al Virology. 2007 Feb 20;358(2):266-72. Epu

Insert

HPV58L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP1SA11

Plasmid

#89162

Purpose

Express Rotavirus SA11 VP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP1

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMX/cyclinB1-GFP (27)

Plasmid

#26061

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-KD LKB1

Plasmid

#8591

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Tags

FLAG

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Article

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-Venus

Plasmid

#20971

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Tags

Venus

Expression

Mammalian

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Promoter

EF1alpha

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS4333 yeTadA1.0-T7 RNAP

Plasmid

#137735

Purpose

pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

Depositor

Christina Smolke

Article

Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

Insert

yeTadA1.0-T7 RNAP

Expression

Bacterial and Yeast

Mutation

E366G (See depositor comment below)

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna3 Flag JIP3b

Plasmid

#53458

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
myc-KIF5B

Plasmid

#127617

Purpose

N-terminal myc-tagged mouse KIF5B

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5B (Kif5b Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FLT3

Plasmid

#23895

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT3 (FLT3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM2991

Plasmid

#40248

Depositor

Susan Golden

Article

Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

Insert

One Step Cloneing Vector for Overexpression

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only
pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSin-EF2-Sox2-Pur

Plasmid

#16577

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ601 - Peft-3 Mos1 transposase

Plasmid

#34874

Purpose

Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Mos1 transpoase

Use

Worm targeting

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL43.1 - Pglh-2::MosTase::glh-2utr

Plasmid

#19332

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pglh-2::Mos1 transposase

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV-10A1

Plasmid

#15805

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

10A1 Murine leukemia virus envelope mRNA

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7851 - 7900 of 19770 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests

More Filters

Applied Filters

Applied Filters

19,770 results