159,481 results

1. pLminP_Luc2P_RE23

   Plasmid

   #90365

   Purpose

   ATF4 - Amino acid deprivation gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   AARE

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV R-CEPIA3mt

   Plasmid

   #140464

   Purpose

   Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

   Insert

   R-CEPIA3mt
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AbVec2.0-IGHG3

   Plasmid

   #99577

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype

   Depositor

   Hedda Wardemann

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-N-Flag-Caspase-1

   Plasmid

   #75128

   Purpose

   Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   caspase 1 (Casp1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P341S in Caspase-1 (please see depositor comments…

   Promoter

   CMV

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWZL Hygro-NMYC TRF2 deltaB deltaM

   Plasmid

   #16355

   Depositor

   Titia de Lange

   Article

   Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

   Insert

   hTRF2 (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

6. HA-GABARAPL1

   Plasmid

   #137759

   Purpose

   Expression of GABARAPL1

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-3HA-His3MX6

   Plasmid

   #41600

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IP Keima-Pex11B

   Plasmid

   #224558

   Purpose

   expresses Keima fused to PEX11B in mammalian cells

   Depositor

   William Kaelin, Gregory Wyant

   Article

   Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

   Insert

   PEX11B (PEX11B Human)
   

   Use

   Retroviral

   Tags

   Keima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

   Plasmid

   #20299

   Purpose

   Cre-activated AAV expression of mCherry

   Depositor

   Karl Deisseroth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pScaf

    Plasmid

    #111401

    Purpose

    Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-P4M-SidM

    Plasmid

    #51471

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Insert

    P4M-SidM(546-647)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-N-R203M

    Plasmid

    #177952

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203M) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-HA-USP3

    Plasmid

    #22582

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP3 (USP3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. RAPpGEX

    Plasmid

    #162241

    Purpose

    Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

    Depositor

    Andreas Faissner

    Article

    Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

    Insert

    lrpap1 (Lrpap1 Mouse)
    

    Use

    Tags

    GST-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmCherryC1 VAP-A

    Plasmid

    #226407

    Purpose

    Expression of human VAP-A fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Use

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

    Plasmid

    #171436

    Purpose

    Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHis-hTG2

    Plasmid

    #100719

    Purpose

    Expression of human transglutaminase 2 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    silent mutation Arg580 (AGG--CGT) to enhance expr…

    Promoter

    T7

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LeGO-dKatushka2

    Plasmid

    #85214

    Purpose

    Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    dKatushka2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BsrGI site removed at 5' end, BsrGI site ins…

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLX302 EGFP-V5

    Plasmid

    #141348

    Purpose

    Expresses V5 tagged GFP

    Depositor

    Kevin Janes

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pS148∙CsR

    Plasmid

    #197858

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3 HA C9ORF72 op

    Plasmid

    #74156

    Purpose

    Express HA tagged optimized C9ORF72 in mammalian cells

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    C9ORF72 (C9orf72 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    optimized sequence

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2474

    Plasmid

    #144029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT3 (ONECUT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDB4695

    Plasmid

    #171127

    Purpose

    A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    OsTIR1-F74A
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF3450

    Plasmid

    #144926

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PM PKA Reporter

    Plasmid

    #104551

    Purpose

    Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Lyn-mTurquoise2-VASP 148 - 164
    

    Use

    Tags

    mTurquoise 2, HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJB06

    Plasmid

    #190480

    Purpose

    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Cas9
    XylR
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    xylR-driven promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mNeon-Vamp2

    Plasmid

    #186619

    Purpose

    Encodes Vamp 2 fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Vesicle Associated Membrane Protein 2 (VAMP2 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #115344

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-NbALFA-degFusionRed

    Plasmid

    #199466

    Purpose

    Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    NbALFA-degFusionRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTriExEKAR2G2

    Plasmid

    #40175

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TFORF2936

    Plasmid

    #144412

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOS (FOS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMT006

    Plasmid

    #64761

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSLIK 3XFLAG-wtGATA6-3XAU1 neo

    Plasmid

    #72618

    Purpose

    Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tight

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

    Plasmid

    #29725

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. TFORF0989

    Plasmid

    #141780

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF9 (KLF9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCMV4Neo-murine IL-17RA-HA

    Plasmid

    #46859

    Purpose

    expresses mouse IL-17RA

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA (Il17ra Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

48. MAC-DPP4

    Plasmid

    #172416

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Dipeptidyl peptidase 4 (DPP4 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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49. CMVdel-dimericTomato-WASp(CRIB)

    Plasmid

    #191450

    Purpose

    Visualizing endogenous & active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WASp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. TFORF2234

    Plasmid

    #143180

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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