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Showing: 7901 - 7950 of 157724 results

  7. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

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  11. Lenti-pNFkB::Cre-mRuby3

    Plasmid
    #164137
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    NFkB promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NFkB synthetic promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164137-plasmid-map-sequence-id-323778

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  20. R777-E137 Hs.MTOR

    Plasmid
    #70421
    Purpose
    Gateway ORF clone of human MTOR [NM_004958.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MTOR (MTOR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70421-plasmid-map-sequence-id-125870

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  26. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Expression
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  30. SSTR4-Tango

    Plasmid
    #66505
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    SSTR4 (SSTR4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66505-plasmid-map-sequence-id-424729

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  33. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid
    #66932
    Purpose
    Mammalian Expression of TRAF2
    Depositor
    Michael Karin
    Insert
    TRAF2 (TRAF2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    66932-plasmid-map-sequence-id-190248

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  37. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  39. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

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  40. TFORF0542

    Plasmid
    #143412
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFAT (ZFAT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143412-plasmid-map-sequence-id-353517

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  47. LSB-hsa-miR-218-5p

    Plasmid
    #103357
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-218-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103357-plasmid-map-sequence-id-195763

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  48. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112285-plasmid-map-sequence-id-227437

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Showing: 7901 - 7950 of 157724 results