167,150 results
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PB-LacI-eGFP
Plasmid#213021PurposepiggyBac donor vector containing LacI-eGFP for random integration by PBaseDepositorInsertLacI**-eGFP
UseMouse Targeting; Piggybase integration (transposo…TagseGFPExpressionMammalianAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry
Plasmid#128327PurposeReporter to evaluate YAP1/TEAD-mediated gene transcriptionDepositorAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6_empty-sgRNA(PP7)
Plasmid#232432Purposeempty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloopDepositorInsertPP7-tagged sgRNA scaffold driven by human U6 promoter
UseCRISPRPromoterU6Available SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-donor_opt(R2Tg)-GFP(intron)
Plasmid#223252PurposeMammalian expression of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
ExpressionMammalianAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIS0
Plasmid#12178Purpose3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein productionDepositorTypeEmpty backboneUseLuciferaseExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_HA1m
Plasmid#190474PurposeExpress the medium-affinity histamine sensor GRAB_HA1m in neuronsDepositorInsertHistamine sensor GRAB_HA1m (medium affinity)
UseAAVPromoterhSynAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas ServiceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-His
Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneTags10xHis and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLOX-TERT-iresTK
Plasmid#12245Purpose2nd generation lentiviral vector for reversible immortalization of human primary cellsDepositorInsertTERT, HSV1-TK (TERT Human)
UseCre/Lox and LentiviralExpressionMammalianMutationLoxP in 3' LTR.Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Giantin_C1
Plasmid#189773PurposeConstruct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusionDepositorInsertmScarlet3-Giantin
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)-c-term-cpGFP-ERexp
Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralExpressionMammalianPromoterCMVd2Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-6a-Ataxin3Q22
Plasmid#22126DepositorAvailable SinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET MBP GFP LIC cloning vector (MBP-msfGFP)
Plasmid#29750DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, SiriusGFP, and TEV c…ExpressionBacterialAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mCherry
Plasmid#41583DepositorHas ServiceCloning Grade DNATypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-YAP1
Plasmid#66853PurposeExpress FLAG-tagged YAP1 in mammalian cellsDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1
Plasmid#188569Purposegreen biosensor for cAMP in neuronsDepositorInsertG-Flamp1
UseAAVTagsHis tagPromoterhSynAvailable SinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0310 TP901-1 Integrase
Plasmid#179110PurposeTP901-1 Integrase expression vectorDepositorInsertTP901-1 Integrase
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-sfGFP
Plasmid#199248PurposeFluorescent labelling of bacterial cells with sfGFPDepositorInsertCodon-optimized sfGFP gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
Plasmid#61593PurposeA single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.DepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTagsHA, NLS, and OLLAS tagExpressionMammalianMutationK175R and K736R (deUb mutations)Available SinceFeb. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-luc2
Plasmid#173006Purposefirefly luciferase reporter vector with 10x clustered UAS binding sitesDepositorInsert10xUAS-luc2
UseLuciferaseExpressionMammalianAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0056
Plasmid#142529PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWXC078
Plasmid#222961PurposeC. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backboneDepositorInsertmScarlet-I3
ExpressionWormAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-DelGFP
Plasmid#133302PurposeEGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA
Plasmid#134925PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE17
Plasmid#90359PurposeNFAT - PKC/Ca++ gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterNFATAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRLsinPGK_IRFP720_WPRE
Plasmid#91785PurposeConstitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.DepositorInsertiRFP720
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-ABE8e-V106W
Plasmid#193653PurposeExpression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-TadA*8e-V106W, EGFP
ExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, TadA*8e-V106WPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Peroxisomes-2
Plasmid#54520PurposeLocalization: Peroxisomes, Excitation: 587, Emission: 610DepositorInsertmCherry-Peroxisomes
TagsPeroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…ExpressionMammalianAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_WT
Plasmid#181920Purpose3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1DepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…ExpressionMammalianPromotertight TRE promoterAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPRC5A-Tango
Plasmid#66382PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…ExpressionBacterialPromoterPA1Available SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)v2.0
Plasmid#186296PurposeA new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.DepositorInserter-(n2)oxStayGold(c4)v2.0
ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Lysosomes-20
Plasmid#56487PurposeLocalization: Lysosome Membrane, Excitation: 485, Emission: 507DepositorInsertLAMP1
TagssfGFPExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRExpressionMammalianAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLRP3-NanoLuc Fusion Vector
Plasmid#237189PurposeExpress NLRP3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMi(3xP3-DsRed)
Plasmid#102539PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed markerDepositorInsertMinos transpoable element with 3xP3-DsRed marker
Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only
