159,589 results
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Plasmid#21222DepositorInsertBrachyury promoter
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF1636
Plasmid#141856PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXC13 (HOXC13 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM28
Plasmid#124960PurposeMammalian expression of Human TRIM28DepositorInserthTRIM28 (TRIM28 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-PALB2
Plasmid#71114Purposemammalian expession of FLAG-PALB2DepositorInsertPALB2 (PALB2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
UseTags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialMutationPromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Ndi-1/myc-His
Plasmid#127503PurposeExpresses myc-tagged yeast Ndi-1 in mammalian cellsDepositorInsertNdi-1 (NDI1 Budding Yeast)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gLacZ.hSyn.flex.H2B.RFP
Plasmid#170373PurposeNegative control guide, Cre-inducible plasmid expressing nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE60-EFG-His
Plasmid#124126PurposeBacterial expression plasmid for PURE system componentsDepositorInsertElongation factor G (fusA Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-eGFP
Plasmid#180143Purposeto make movies of LRP6 vesiclesDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-L26A,F27A
Plasmid#89084Purposemammalian expression of human androgen receptor with mutation of N-terminal FxxLF motifDepositorInserthAR FxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-L26A,F27APromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gamillus/ pcDNA3
Plasmid#124837Purposemammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent proteinDepositorInsertGamillus
UseTagsNo tagExpressionMammalianMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC20A1_STOP
Plasmid#161176PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC20A1 (SLC20A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR8/GW:U6
Plasmid#85981PurposeGolden Gate Level 0 cassette encoding the U6 promoterDepositorInsertT. pseudonana U6 promoter
UseCRISPR; Diatom (t. pseudonana)TagsExpressionMutationPromoternoneAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
psubCAG-WPRE-hVEGF-D-Strept
Plasmid#119227PurposePlasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorInsertVEGFD (VEGFD Human)
UseAAVTagsStreptExpressionMutationPromoterCAGAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM1192
Plasmid#115683PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertmCherry, aac(3)IV, tsr
UseTagsStrep-tagII on mCherry, codon optimizedExpressionBacterialMutationmCherry codon optimized for StreptomycesPromoterSF14*Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-26:ST6GAL1-mEGFP
Plasmid#101786PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1DepositorInsertST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BoxB
Plasmid#92004PurposeExpresses firefly luciferase with BoxB sequences in 3' UTRDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-MSL3L1
Plasmid#29761DepositorInsertMSL3L1 (MSL3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 tTA UQCRC2
Plasmid#177978PurposeAll-in-one dox-repressible expression of cDNADepositorInsertUQCRC2 (UQCRC2 Human)
UseLentiviralTagsExpressionMammalianMutationSilent point mutations to UQCRC2 sgRNA targetPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-hAR-W433A,L436A,F437A
Plasmid#89086Purposemammalian expression of human androgen receptor with mutations of N-terminal WxxLF motifDepositorInserthAR WxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-W433A,L436A,F437APromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR2
Plasmid#184047PurposeImproved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.DepositorInsertExRai-AktAR2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-YAP1
Plasmid#66853PurposeExpress FLAG-tagged YAP1 in mammalian cellsDepositorInsertYAP1 (YAP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_RGECO1.2
Plasmid#164592PurposeStable integration and expression of RGECO1.2 in mammalian cellsDepositorInsertR-GECO1.2
UseLentiviralTagsExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-L26A,F27A,W433A,L436A,F437A
Plasmid#89085Purposemammalian expression of human androgen receptor with mutations of N-terminal FxxLF and WxxLF motifsDepositorInserthAR FxxLF WxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-L26A,F27A,W433A,L436A,F437APromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-F23A
Plasmid#89089Purposemammalian expression of human androgen receptor with mutation of N-terminal FxxLF motifDepositorInserthAR-FxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-F23APromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
dsRed-cent2
Plasmid#29523DepositorInsertCent2 (CETN2 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2
Plasmid#17218PurposeRetroviral expression of human SOX2DepositorInsertSOX2 (SOX2 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
psubCMV-mVEGF-D-WPRE
Plasmid#119226PurposePlasmid encodes full-length (unprocessed) murine VEGF-D to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorInsertVegfd (Vegfd Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650D
Plasmid#171246PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)DepositorInsert3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Asp (AR-S650D)PromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650A
Plasmid#171245PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)DepositorInsert3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Ala (AR-S650A)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_ATP1.0mut
Plasmid#167580PurposeExpress the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytesDepositorInsertATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-GFP-T(WT)
Plasmid#181736Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-SLC26A6_STOP
Plasmid#161222PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC26A6 (SLC26A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGSHU
Plasmid#72244PurposeContains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
hyg
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHmScarlet
Plasmid#166890PurposeFor vesicle exocytosis visualization using pHmScarletDepositorInsertVAMP2 (Vamp2 Mouse)
UseTagspHmScarletExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only