8,883 results

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Fubi-ChR2-GFP

Plasmid

#22051

Purpose

Viral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

UbC

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pDest-544

Plasmid

#11519

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway destination vector

Tags

His6-NusA

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pBad-mAmetrine1.1

Plasmid

#18084

Purpose

Bacterial expression of mAmetrine (version 1.1) fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine1.1

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
HA-merlin

Plasmid

#32836

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Merlin (NF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
C32V

Plasmid

#26396

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-32-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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Flag TSC2 wt

Plasmid

#12132

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB deltaM

Plasmid

#16069

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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L4417

Plasmid

#1649

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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V249 pCEP-4HA B56delta

Plasmid

#14536

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5D B56 delta regulatory subunit of PP2A (PPP2R5D Human)

Tags

4 HA

Expression

Mammalian

Mutation

R210H

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pAKlux1

Plasmid

#14073

Depositor

Attila Karsi

Article

Karsi et al FEMS Microbiol Lett. 2006 Jul. 260(2):21

Insert

Bacterial luciferase

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
RGS-SENP3

Plasmid

#18048

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP3 (SENP3 Human)

Tags

RGS

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-RosaR4 KKR mutations

Plasmid

#37199

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to RosaR4 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs R4, and SV40 NLS

Expression

Mammalian

Mutation

I538K, E490K, and H537R

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate2-P2A-APEX2-2xFYVE_hrs

Plasmid

#67663

Purpose

Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC1124

Plasmid

#41560

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-wild type mKdm2b-Flag

Plasmid

#61739

Purpose

Express wild-type mouse histone demethylase Kdm2b

Depositor

Yi Zhang

Article

He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

Insert

Kdm2b (Kdm2b Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only
PKC beta I DN

Plasmid

#16381

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-delta90GFP

Plasmid

#26645

Depositor

Anjen Chenn

Article

Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Insert

delta90 beta-catenin (Ctnnb1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPGAL4.2::p65Uw

Plasmid

#26229

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::p65 (RELA Human, Budding Yeast)

Expression

Insect

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/HA-FLAG RBP2 H483A

Plasmid

#14801

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Changed His 483 to Ala, R46W

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE zeo Ecotropic Receptor

Plasmid

#10687

Depositor

William Hahn

Insert

Ecotropic Receptor (Slc7a1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
MYD88 flag

Plasmid

#13093

Depositor

Ruslan Medzhitov

Insert

MYD88 (Myd88 Mouse)

Tags

flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-I-N-CAAX

Plasmid

#41686

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145N-IntersectinDH-Dronpa145N-CAAX

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEC19

Plasmid

#30178

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Crimson

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p416 103Q GPD

Plasmid

#1180

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pYESir2HY-puro

Plasmid

#1770

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

H363Y, residue 363 changed from histidine to tyro…

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pClusterin-ERT2CreERT2

Plasmid

#14796

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD285

Plasmid

#66826

Purpose

mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKACA

Plasmid

#23495

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACA (PRKACA Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
p210 pCMV-CREM

Plasmid

#8395

Purpose

Cre with chimeric/β-globin intron within the Cre coding sequence

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

CMV

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-K27 histone methylation reporter

Plasmid

#22865

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K27 HMR

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
Clathrin-LCa-EYFP

Plasmid

#21741

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pBABE-neo largeTgenomic

Plasmid

#10891

Depositor

William Hahn

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-XIAP

Plasmid

#8340

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

GST

Expression

Bacterial

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Article

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

mVenus-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pBJ5-CnA

Plasmid

#11785

Depositor

Anjana Rao

Article

Clipstone et al J Biol Chem. 1994 Oct 21. 269(42):26431-

Insert

CnA (Ppp3ca Mouse)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
3279 pSFFV-neo Bid human

Plasmid

#8766

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid ORF cDNA FL (BID Human)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-mTOR (1271-2008 aa)

Plasmid

#21746

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1271-2008) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1271-2008

Available Since

Aug. 18, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-OT (wt TCF-4 sites)

Plasmid

#16558

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of wildtype Tcf-4 binding sites

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

Plasmid

#19326

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pmyo-3::twk-18(cn110)cDNA

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,883 results