19,200 results
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Plasmid#87218Purposelentiviral expression of ODD-EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationODD domainPromoterEF1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
IRES-puro-GFP
Plasmid#16616DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNb30_opt.IRES.eGFP
Plasmid#22493DepositorInsertCodon optimised Calcineurin B mutant 30 (PPP3R1 Human)
UseRetroviralTagsExpressionMutationL124T; K125-LA-InsPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRX-No-HaloTag7-mGFP-LC3-mRFP
Plasmid#184902PurposeStable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic fluxDepositorInsertLC3B
UseTagsHaloTag7, monomeric EGFP, and monomeric RFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato
Plasmid#60658PurposeFlp-dependent expression of mNaChBac-T2A-tdTomatoDepositorHas ServiceAAV8InsertFLEX-mNaChBac-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pseudojanin (PJ)
Plasmid#37999DepositorUseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
CBF:H2B-Venus
Plasmid#44211DepositorInsertCBF1-Responsive-Element
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSC101-pT7-T3RNAP
Plasmid#140872PurposeT7 promoter driven synthesis of T3 RNA polymeraseDepositorInsertT3 RNA polymerase
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ND6-DdCBE-left side TALE
Plasmid#157842Purposeexpresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1
Plasmid#19838DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
nls-NgAgo-GK
Plasmid#78253PurposeMammalian expression of NgAgo (Argonaute from N. gregoryi).DepositorInsertNgAgo
UseTagsa nuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby2-SpHis5
Plasmid#44858PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selectionDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RLuc
Plasmid#83282Purposerecombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)DepositorInsertRenilla Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IN
Plasmid#60612PurposeBackbone for tet-inducible genesDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b
Plasmid#111621PurposeExpression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cellsDepositorInsertExonuclease 1b (EXO1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS9counter
Plasmid#107192PurposeExpresses CAS9 and sgRNA for targeted counterselection in S. aureusDepositorInsertsCas9
sgRNA
UseTagsExpressionBacterialMutationPromoterprab17Available sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-hTRIM9
Plasmid#51032Purposeentry vector for cloning human TRIM9DepositorInsertTRIM9 (TRIM9 Human)
UseEntry vectorTagsExpressionMutationPromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:NICD-P2A-mRFP
Plasmid#127594PurposeZebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.DepositorInsertNICD (notch1a Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3113 GST-p53
Plasmid#10852DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pLXSN18E6E7
Plasmid#53459PurposeThe plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.DepositorInsertHPV18E6E7
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
spCas9-BlastR (pCBhCas9-BlastR)
Plasmid#71489PurposeExpresses SpCas9 and Blasticidin resistanceDepositorInsertspCas9
UseTagsExpressionMutationPromoterCBhAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-p150Glued
Plasmid#36154DepositorInsertp150Glued (DCTN1 Human)
UseTagsEGFPExpressionMammalianMutationsee documentationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Nanog-EGFP
Plasmid#21321PurposeLentiviral vector, expresses EGFP under Nanog promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterNanog promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXC1
Plasmid#153114PurposeExpression of Flag tagged human FOXC1 in mammalian cellsDepositorInserthuman FOXC1 (FOXC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Duet011
Plasmid#17627DepositorInsertUBC promoter - GFP - PGK promoter - Hygro
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PE2-C
Plasmid#164908PurposeExpresses the C-terminal split-intein fragment of PE2DepositorInsertPE2-C
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SEP-GluA1 (M1)
Plasmid#64942Purposemammalian expression of rat GluA1DepositorInsertGluA1 (Gria1 Rat)
UseTagsSEP (superecliptic pHluorin)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×2-N1
Plasmid#86775PurposeA mammalian expression vector with double EGFP at C-terminus.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSVL(D2m)
Plasmid#29336DepositorInsertDimer of HDV genome with 2 nucleotide deletion in delta antigen
UseTagsExpressionMammalianMutationContains a dimer of the complete HDV genome excep…PromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCB-hDot1Lwt
Plasmid#74173PurposeFor packaging hDot1Lwt to retrovirus.DepositorInserthDot1L (DOT1L Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
p201G Cas9
Plasmid#59178PurposeCas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
sGFP
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-PI3K CAR
Plasmid#113018Purposefor lentivirus production of CD19-PI3K CARDepositorInsertCD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Synthetic, Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
psd44-Lactadherin46
Plasmid#46830Purposeexpression of human lactadherin in mammalian cellsDepositorInsertMFGE8 (MFGE8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA
Plasmid#163024PurposeExpression of CRISPRi with U6-gRNA (Bsa1 sites)DepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 polyA, and myc NLSExpressionMammalianMutationPromoterhEF-1aAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFPd2
Plasmid#14760DepositorInsertEGFPd2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C2 Chfr (1014)
Plasmid#61853Purposemammalian expresion of Chfr fused to EGFPDepositorInsertChfr (CHFR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-N1
Plasmid#54617PurposeLocalization: N1 Cloning Vector, Excitation: 384, Emission: 450DepositorTypeEmpty backboneUseTagsmAzuriteExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorInsertYAP responsive promoter (YAP1 Human)
UseLentiviralTagsDsRED-DHFRExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only