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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,206 results

Showing: 9701 - 9750 of 19206 results

  4. pcDNA-GFP-STAT1

    Plasmid
    #11987
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    no mutations
    Promoter
    Available since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  5. mCherry-CDC42-C-10

    Plasmid
    #55014
    Purpose
    Localization: Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CDC42 (CDC42 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Article
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hUBC promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

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  9. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-164306

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  16. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Article
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Phage, Human, Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40591-plasmid-map-sequence-id-302078

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  33. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  34. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Use
    Tags
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66190-plasmid-map-sequence-id-169495

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  39. pL99

    Plasmid
    #78577
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    inducible PnitA-NitR system
    Available since
    May 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78577-plasmid-map-sequence-id-263121

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  44. pLenti CMVtight Puro DEST (w768-1)

    Plasmid
    #26430
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Lentiviral; Tet-on advanced destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    26430-plasmid-map-sequence-id-12473

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  47. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    James Thomson, Erik Sontheimer
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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Showing: 9701 - 9750 of 19206 results