166,224 results

1. [#GS16-LV] Syn1-MitoTRACER - Donor

   Plasmid

   #233565

   Purpose

   Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   MitoTRACER-Donor
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   GFP11 and HA Tag

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

   Plasmid

   #154868

   Purpose

   Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

   Depositor

   Ulrik Gether

   Has Service

   AAV Retrograde and AAV8

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   Human Synapsin-1

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT3-EF1A-mTert

   Plasmid

   #162555

   Purpose

   Overexpression of Tert

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   Overexpression of Tert (Tert Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF1A

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-syn-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234438

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Has Service

   AAV1

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.2/NS1

   Plasmid

   #120824

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS1 ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSV-Rev

   Plasmid

   #12253

   Purpose

   3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Article

   Dull et al J Virol. 1998 Nov . 72(11):8463-71.

   Insert

   Rev (rev )
   

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-tevopreq1-GG-acceptor

   Plasmid

   #174038

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMDLg/pRRE

    Plasmid

    #12251

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    HIV-1 GAG/POL
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG RpH-LAMP1-3xFLAG

    Plasmid

    #163018

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe EGFR(L858R/T790M)

    Plasmid

    #32073

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L858R/T790M

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge