161,488 results

1. ER-SPLICSs-cFAST10

   Plasmid

   #210431

   Purpose

   Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   ER-SPLICSs-cFAST10
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF6a-CD28-PafA

   Plasmid

   #113400

   Purpose

   Expresses CD28-PafA in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   CD28-PafA
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SPLICS Mt-ER- PM Long P2A

   Plasmid

   #164109

   Purpose

   Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP PM-Mt-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG1169 FIREmate-ECFP-Cb5

   Plasmid

   #205167

   Purpose

   Expresses FIREmate-ECFP-Cb5 in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Cb5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SPLICSFAST-LDh-MT-P2A

   Plasmid

   #214896

   Purpose

   Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LDh-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SPLICS Mt-ER- PM Short P2A

   Plasmid

   #164110

   Purpose

   Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP PM- Mt-ER Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SPLICSFAST-LY-MT-P2A

   Plasmid

   #214897

   Purpose

   Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LY-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SPLICSFAST-LDh-LY-P2A

    Plasmid

    #214895

    Purpose

    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LDh-LY-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SPLICS Mt-ER- Po Short P2A

    Plasmid

    #164118

    Purpose

    Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP Mt-ER- Po Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SPLICSFAST-PO-MT-P2A

    Plasmid

    #214899

    Purpose

    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-PO-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-TRE3G-BCCP-PupE-IRES-BFP

    Plasmid

    #113405

    Purpose

    Express inducible BCCP-PupE in mammalian cell

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    BCCP-PupE-IRES-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPLICSFAST-ER-MT-P2A

    Plasmid

    #214892

    Purpose

    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. V5-LOV-Turbo1-NIK3x_pTRE-G418

    Plasmid

    #202017

    Purpose

    expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-LOV-Turbo1-NIK3x
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPLICSFAST-NU-MT-P2A

    Plasmid

    #214898

    Purpose

    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-NU-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SPLICS Mt-ER- Po Long P2A

    Plasmid

    #164117

    Purpose

    Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP Mt-ER- Po Long P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG1171 FIREmate-ECFP-Giantin

    Plasmid

    #205166

    Purpose

    Expresses FIREmate-ECFP-Giantin in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSV-Rev

    Plasmid

    #12253

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    Rev (rev )
    

    Use

    Lentiviral; Packaging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge