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Showing: 10451 - 10500 of 162536 results

  5. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  6. pDGE333

    Plasmid
    #153242
    Purpose
    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153242-plasmid-map-sequence-id-305781

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  17. pHRIG-Akt3

    Plasmid
    #53596
    Purpose
    lentiviral expression of constitutively active human Akt3 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt3 (AKT3 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pLenti6-V5-Topo mCherry

    Plasmid
    #128062
    Purpose
    Lentiviral vector for C terminal mCherry fusions
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128062-plasmid-map-sequence-id-403501

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  25. pLenti-MetGFP

    Plasmid
    #37560
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    37560-plasmid-map-sequence-id-339875

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  27. pBbB8k-csg-NbGFP

    Plasmid
    #166858
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166858-plasmid-map-sequence-id-329027

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  28. pET-EIN-FTO(32-505)

    Plasmid
    #186466
    Purpose
    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    Fat mass obesity associated protein (FTO Human)
    Use
    Tags
    6 Histidine-EIN (N-terminal domain of E.coli Enzy…
    Expression
    Bacterial
    Mutation
    amino acid 32-505
    Promoter
    T7
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186466-plasmid-map-sequence-id-365741

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  31. pBAD-GINKO2

    Plasmid
    #177116
    Purpose
    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2
    Depositor
    Robert Campbell
    Article
    Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do
    Insert
    GINKO2
    Use
    Tags
    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    177116-plasmid-map-sequence-id-348390

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  37. ADRA2C-Tango

    Plasmid
    #66218
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2C (ADRA2C Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. MYST3

    Plasmid
    #25181
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST3_13 (KAT6A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains catalytic region ( residues 497-780).
    Promoter
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Article
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Promoter
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171098-plasmid-map-sequence-id-336112

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  46. pSG5 PPAR alpha

    Plasmid
    #22751
    Depositor
    Bruce Spiegelman
    Insert
    PPARalpha (Ppara Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    22751-plasmid-map-sequence-id-160481

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  47. CROPSeq-PP7-p65-HSF

    Plasmid
    #196719
    Purpose
    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196719-plasmid-map-sequence-id-388066

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  48. TFORF0924

    Plasmid
    #141757
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR2F2 (NR2F2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141757-plasmid-map-sequence-id-392752

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  50. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

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Showing: 10451 - 10500 of 162536 results