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We narrowed to 13,968 results for: SHI

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Showing: 10801 - 10850 of 13968 results

  10. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  28. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  41. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  42. pAVA-Gal11p-LGF2(fs)

    Plasmid
    #127482
    Purpose
    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    Expression
    Bacterial
    Mutation
    LGF2 is frame shifted by the insert of one nucleo…
    Available Since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127482-plasmid-map-sequence-id-262919

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Showing: 10801 - 10850 of 13968 results