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173,273 results

Showing: 11051 - 11100 of 173273 results

  1. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab18
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166431-plasmid-map-sequence-id-333545

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  3. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  9. pAc5.1B-DmPABPC1-V5His6_D

    Plasmid
    #146093
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Expression
    Insect
    Mutation
    one non-silent mutation G26S compared to the sequ…
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146093-plasmid-map-sequence-id-362948

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  12. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

    Plasmid
    #138368
    Purpose
    Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138368-plasmid-map-sequence-id-272014

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  14. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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  26. TV_ANXA1-p2a-mScarlet-nls-p2a-icasp9_pgk-blast

    Plasmid
    #251171
    Purpose
    Targeting vector for C-terminal knockin at ANXA1 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9.
    Depositor
    Hugo Snippert
    Insert
    p2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human ANXA1 (ANXA1 Human)
    Use
    CRISPR
    Tags
    p2a-mScarlet-nls-p2a-icasp9
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251171-plasmid-map-sequence-id-500693

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  27. TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251173
    Purpose
    Targeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251173-plasmid-map-sequence-id-500690

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  34. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(WT) (DRR1012)

    Plasmid
    #217799
    Purpose
    Variant CE1 construct with wild-type M-MLV revese transcriptase (RT), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217799-plasmid-map-sequence-id-432866

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  44. pQCXIP-Laforin Myc W32G

    Plasmid
    #84672
    Purpose
    mammalian expression and/or retrovirus preparation
    Depositor
    Ayano Satoh
    Article
    Toyota et al Sciencematters
    Insert
    Laforin (EPM2A Human)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    5'LTR
    Available Since
    Nov. 24, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. pCAG-VSVM

    Plasmid
    #64086
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene
    Depositor
    Ian Wickersham
    Insert
    VSV matrix protein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64086-plasmid-map-sequence-id-107218

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  48. DHX9A

    Plasmid
    #42456
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DHX9A (DHX9 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  49. SIN-cPPT-PGK-mitoGO-ATeam2-WPRE

    Plasmid
    #245083
    Purpose
    Mitochondrial ATP biosensor
    Depositor
    Nicole Déglon
    Insert
    mitoGO-ATeam2
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Mouse PGK
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    245083-plasmid-map-sequence-id-499134

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  50. pMSCV-IRES-Blue FP

    Plasmid
    #52115
    Purpose
    a bicistronic IRES-Blue FP containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-Blue FP
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52115-plasmid-map-sequence-id-351562

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Showing: 11051 - 11100 of 173273 results