159,557 results

1. pDestTol2pACryGFP

   Plasmid

   #64022

   Purpose

   Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

   Depositor

   Peter Currie, Joachim Berger

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   GFP
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   alpha-Acrystallin promoter

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-BR1

   Plasmid

   #160333

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   BR1
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMEH343 CD63 MS2 HA

   Plasmid

   #168217

   Purpose

   Mammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   CD63 (CD63 Human)
   

   Use

   Tags

   HA and MS2 dimer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

   Plasmid

   #138315

   Purpose

   Lenti expression of mTgBFP2

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   mTAGBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-PIK3CA

   Plasmid

   #116771

   Purpose

   Lentiviral expression of PIK3CA

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CMV-Pink Flamindo-p2a-bPAC

   Plasmid

   #202716

   Purpose

   Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAF0690 Ef1a-Kp03_attB-BFP acceptor

   Plasmid

   #193473

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03_attB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-Calnexin-N-14

   Plasmid

   #55005

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCBFD2Bad

    Plasmid

    #53574

    Purpose

    Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LRP6-pCS2

    Plasmid

    #27242

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tdTomato-CD9-10

    Plasmid

    #58076

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 Flag BimL

    Plasmid

    #24233

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bim L (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. Twinkle-APEX2-V5

    Plasmid

    #129705

    Purpose

    APEX2 fused to the mitochondrial nucleoid protein Twinkle

    Depositor

    Alice Ting

    Article

    Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

    Insert

    Twinkle-APEX2-V5 (TWNK, APEX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LeGO-C2

    Plasmid

    #27339

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR-mKate2

    Plasmid

    #228610

    Purpose

    Mammalian expression of mKate2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

    Insert

    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-GRET

    Plasmid

    #158222

    Purpose

    Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    GFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a-C2-mKate

    Plasmid

    #208763

    Purpose

    To express C2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMHE-vhhGFP4-hIgG1-Fc

    Plasmid

    #105576

    Purpose

    T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    hSyn

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-BICDN-CIBN

    Plasmid

    #102254

    Purpose

    Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    GFP-BICDN-CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR221_SLCO1B1

    Plasmid

    #132188

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO1B1 (SLCO1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. p5E-kdrl

    Plasmid

    #78687

    Purpose

    kdrl promoter for Gateway cloning

    Depositor

    Sarah Childs, Jau-Nian Chen

    Article

    Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

    Insert

    kdrl promoter (kdrl Zebrafish)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    kdrl

    Available since

    June 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET MBP mCherry LIC cloning vector (MBP-mCherry)

    Plasmid

    #29747

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF2314

    Plasmid

    #142029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RXRB (RXRB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CXCR5-DuET

    Plasmid

    #213222

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR5 (CXCR5 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMAH-POLY-eRF1(E55D)

    Plasmid

    #157925

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37090

    Purpose

    Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AnkyrinG-190-GFP

    Plasmid

    #31059

    Depositor

    Vann Bennett

    Article

    Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

    Insert

    Ankyrin G (Ank3 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMIG-Aalpha WT

    Plasmid

    #10884

    Depositor

    William Hahn

    Article

    Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

    Insert

    PP2A Aalpha (PPP2R1A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Plenti-ARE-HSVTK-luc

    Plasmid

    #161789

    Purpose

    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

    Depositor

    Hans-Guido Wendel

    Article

    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

    Inserts

    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and Murine Gclc ARE

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA5/FRT/TO V5 HSPA2

    Plasmid

    #19512

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 2 (HSPA2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SpRYc

    Plasmid

    #175575

    Purpose

    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

    Insert

    SpRYc
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    PID of SpRY grafted onto the N-terminal domain of…

    Promoter

    CMV

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PLEX_313 SLC31A1

    Plasmid

    #184493

    Purpose

    Lentivirus expression for SLC31A1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    SLC31A1 (SLC31A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBbA5a-sufABCDSE

    Plasmid

    #195056

    Purpose

    Expresses SUF operon from E. coli from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufABCDSE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. DsRed2-Peroxisomes-4

    Plasmid

    #54503

    Purpose

    Localization: Peroxisomes, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    DsRed2 and Peroximal Targeting Signal 1 (PTS1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pLminP_Luc2P_RE53

    Plasmid

    #90398

    Purpose

    STAT1 - STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Golgi-TurboID

    Plasmid

    #200959

    Purpose

    Transiently expresses golgi localized TurboID with HA tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAX2

    Plasmid

    #117398

    Purpose

    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUB-GFP

    Plasmid

    #11155

    Purpose

    Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    Ubiquitin C promoter (UBC Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pQE30-hPolB

    Plasmid

    #70761

    Purpose

    bacterial expression of human DNA Polymerase beta (POLB)

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    POLB (POLB Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    R333L

    Promoter

    T5/lac operon

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. mPA-GFP-LaminB1-10

    Plasmid

    #57141

    Purpose

    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

48. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. 2Bc-T MBP_BoMoC(ed)_6xH

    Plasmid

    #186461

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPLV_C1

    Plasmid

    #186415

    Purpose

    Endogenous murine RNase inhibitor production for cell-free lysate

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

    Insert

    Murine RNase Inhibitor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge