We narrowed to 18,651 results for: Met
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Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP3
Plasmid#75165PurposeLentiCRISPR-EGFP with sgRNA targeting human RIP3DepositorInsertRIP3 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-ovoD1
Plasmid#111142PurposeExpresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogasterDepositorAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA3
Plasmid#113132PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA-mut
Plasmid#140555PurposeExpresses the DA-insensitive control sensor GRAB_DA-mut in neuronsDepositorHas ServiceAAV9InsertDA-insensitive sensor GRAB_DA-mut (control sensor)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-PguCas13b-msfGFP-NES-Flag
Plasmid#165072Purposeoverexpression of PguCas13b in human cellsDepositorInsertPguCas13b
UseLentiviralExpressionMammalianAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA4
Plasmid#113133PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, Synthetic Biology, and TALENExpressionMammalianAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA2
Plasmid#113131PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTDH-His-bio
Plasmid#52331PurposeExpresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertMTDH (MTDH Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-NS1NF
Plasmid#175279PurposeAAV vector mediating inducible expression of the NS1 gene of YFV-17DDepositorAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRVL-6
Plasmid#104584PurposeContains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functionsDepositorInsertHuman IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
ExpressionMammalianMutationL234A/L235E/G237A and K322A/P331SPromoterCMVAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
TagsV5 Epitope TagExpressionMammalianPromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-vDIO-mScarlet-IRES-tTA
Plasmid#118030PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-vDIO-mScarlet-IRES-tTA
UseAAVPromoterTREAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-AgDD
Plasmid#80625Purposecreate nuclear protein aggregates in mammalian cellsDepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
anti-FX(a) Emi HC
Plasmid#113665PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FX/FXa heavy chain
ExpressionMammalianPromoterCMV PromoterAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-muGFP
Plasmid#166727PurposeAAV, encodes monomeric ultrastable GFP (muGFP)DepositorInsertmuGFP
UseAAVPromoterChicken Beta ActinAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ADARcd-YTHD422N
Plasmid#194402PurposeADAR1 catalytic domain E488Q fused to YTHD422NDepositorInsertADARcd fused to YTHD422N (ADAR Human)
TagsADARcd-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2attB-FST344-HA-CPL
Plasmid#131460PurposeEncodes human Follistatin proteinDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-mBaoJin-N1
Plasmid#229567PurposeExpresses P450-mBaoJin fusion in mammalian cellsDepositorInsertmBaoJin
ExpressionMammalianAvailable SinceJuly 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEJS887_pTetR-P2A-BFPnls/sgAlpha
Plasmid#108651PurposeAlpha satellite repeats targeting Spy sgRNA under U6 promoterDepositorInsertsAlpha satellite repeats targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NES-NIR-GECO1
Plasmid#113683PurposeExpress NIR-GECO1 preferentially in neuronsDepositorInsertNES-NIR-GECO1
UseAAVPromoterhSynAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2h
Plasmid#208721PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVPromoterhSynAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 Luc2-IRES-GFP
Plasmid#224467PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFPDepositorInsertLuciferase
UseLentiviralPromoterEF1aAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-GEX-GECO1
Plasmid#32443PurposeMammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEX-GECO1.0
ExpressionMammalianMutationGCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔPRYSPRY
Plasmid#105524PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Flag-SAMTOR
Plasmid#102420Purpose3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoterDepositorAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
anti-FIX(a) Emi HC
Plasmid#113664PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FIX/FIXa heavy chain
ExpressionMammalianPromoterCMV PromoterAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KQ
Plasmid#17562DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
TagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable SinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP3
Plasmid#160086PurposeBacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKADepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TP5
Plasmid#160088PurposeBacterial expression of Tn5-APEX2 fusion protein with linker GSGAGADepositorInsertTn5 transposase/APEX2 peroxidase fusion protein
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-pEGFP-C1
Plasmid#177408PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.DepositorInsertBcl-XL
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP C1
Plasmid#202424PurposeEncodes GFP aloneDepositorTypeEmpty backboneUseLentiviralAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NS3-NLS/VPR
Plasmid#112244PurposeEncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.DepositorInsertdCas9-NS3-NLS/VPR
TagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(A)
Plasmid#87913PurposeExpresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro/HA-FLAG-RBP2
Plasmid#14802DepositorInsertRetinoblastoma Binding Protein 2 (KDM5A Human)
UseRetroviralTagsFLAG and HAExpressionMammalianAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoECDS
Plasmid#41848DepositorAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
TagsGFP (S65C)ExpressionWormPromotersur-5Available SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorInserthA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-PUM2-rABE-Flag
Plasmid#191386PurposeDoxycycline inducible expression of PUM2-rABEDepositorInsertPUM2-rABE (PUM2 Human, Synthetic)
UseLentiviralTagsFlagExpressionMammalianPromotertet-inducible promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-USP11
Plasmid#78849PurposeTo purify GST-tagged recombinant USP11 proteinDepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK2
Plasmid#199641PurposeExpresses VRK2DepositorInsertVRK2 (VRK2 Human)
UseLentiviralAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe human FOXC2
Plasmid#15535DepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL(D523G)
Plasmid#66837PurposeExpression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.DepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-LwaCas13a-msfGFP-NES-Flag
Plasmid#165070Purposeoverexpression of LwaCas13a in human cellsDepositorInsertLwaCas13a
UseLentiviralExpressionMammalianAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC-] (AVA2515)
Plasmid#85443PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(D)
Plasmid#87914PurposeExpresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only