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161,407 results

Showing: 11951 - 12000 of 161407 results

  1. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid
    #136348
    Purpose
    Lentiviral inducible expression (TetOn) of human BIRC5
    Depositor
    Guillermo de Cárcer
    Insert
    BIRC5 (BIRC5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136348-plasmid-map-sequence-id-270811

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  8. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  13. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Article
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103056-plasmid-map-sequence-id-199055

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  16. pAC574

    Plasmid
    #127975
    Purpose
    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
    Insert
    hygB (hygromycin resistance marker)
    Use
    Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans trpC promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    127975-plasmid-map-sequence-id-254311

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  17. pUC19-link-mCherry-FLAG:cat

    Plasmid
    #211831
    Purpose
    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.
    Depositor
    Peng Chen
    Insert
    mCherry-FLAG-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    211831-plasmid-map-sequence-id-418058

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  19. pAAVdual-CMV-eGFP

    Plasmid
    #230931
    Purpose
    pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.
    Depositor
    Qizhao Wang
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230931-plasmid-map-sequence-id-458357

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  33. pTK194

    Plasmid
    #59563
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP XbaI attB1 Kozak mKate opal attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59563-plasmid-map-sequence-id-92968

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  35. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    134843-plasmid-map-sequence-id-266705

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  37. pSF3-microID

    Plasmid
    #172880
    Purpose
    expresses microID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    microID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172880-plasmid-map-sequence-id-340538

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  38. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  39. TFORF1207

    Plasmid
    #144089
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CASZ1 (CASZ1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144089-plasmid-map-sequence-id-396173

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  40. pET9d-hnRNP-A1

    Plasmid
    #23026
    Depositor
    Douglas Black
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 26, 2012
    Availability
    Academic Institutions and Nonprofits only
    23026-plasmid-map-sequence-id-148002

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  43. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  44. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    87836-plasmid-map-sequence-id-176310

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Showing: 11951 - 12000 of 161407 results