We narrowed to 167,150 results for: Gene
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Plasmid#48225PurposedCas9VP160 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPRTagsHA Tag and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
proE-cad178-Luc
Plasmid#42081DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseExpressionMammalianMutationcontains E-cadherin promoter region from -178 to …PromoterE-cadherinAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR
Plasmid#182249PurposeHuman HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of HNRNPAB gene
UseLuciferaseExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-BICRAL-FLAG-pgk-puro
Plasmid#107732PurposeTet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORFDepositorAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianPromoterAlb-AATAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)
Plasmid#85038PurposeFor Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.DepositorInsertFRT-stop-FRT
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)
Plasmid#133801PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA4
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianPromoterhUbCAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyPromoterhPGK promoterAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP
Plasmid#199456PurposeExpression of stdPCP-PH in mammalian cellsDepositorInsertstdPCP-PH
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster vasaAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eYFP
Plasmid#117383PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFPDepositorHas ServiceAAV1InsertEYFP
UseAAVExpressionMammalianPromoterTREAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)
Plasmid#133802PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA5
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
DSCAM_pCSdest
Plasmid#53906DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
E1b-GFP-Tol2-Gateway
Plasmid#37846DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorPromoterE1bAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
proE-cad670-Luc
Plasmid#42083DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseExpressionMammalianMutationcontains E-cadherin promoter region from -670 to …PromoterE-cadherinAvailable SinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
CCT8_pCSdest
Plasmid#53795DepositorAvailable SinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)
Plasmid#129668PurposeA repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugABE-NNG-P2A-puro
Plasmid#214354PurposeExpresses SlugABE-NNG and puromycin resistance genesDepositorInsertSlugABE-NNG and puromycin resistance genes
ExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-NeoR-LoxP
Plasmid#227503PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)
Plasmid#134341PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIC3
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-DDdCas9VP192-T2A-EGFP-ires-puro
Plasmid#69534PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.DepositorInsertDD-dCas9VP192-T2A-EGFP
TagsC-term T2A-EGFP and DHFR Destabilised DomainExpressionMammalianPromoterCAGAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
ExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…Available SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADAMTS1_pCSdest
Plasmid#53807DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
ExpressionBacterialPromoterXyl/tet promoter that is constitutively activeAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PC3-709-33-10_GAL4-FKBP12(F36V)
Plasmid#200438PurposeExpresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target proteinDepositorInsertGAL4-FKBP12(F36V)
TagsGAL4, FKBP12(F36V)ExpressionMammalianAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Equalizer-L episome
Plasmid#169738PurposeEqualizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.DepositorInsertmiR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mNeonGreen
Plasmid#99132PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreenDepositorInsertDIO-mNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-MSK1
Plasmid#165601PurposeExpresses Sp dCas9 fused to human MSK1DepositorInsertribosomal protein S6 kinase A5 (RPS6KA5 S. Pyogenes, Synthetic, Human)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianPromoterEF1aAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCas9-NNG-P2A-puro
Plasmid#214352PurposeExpresses SlugCas9-NNG and puromycin resistance genesDepositorInsertSlugCas9-NNG and puromycin resistance genes
ExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BMPS HK318/9EE-6xHis
Plasmid#204836PurposeMammalian expression of recombinant BMPS WTDepositorAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL005
Plasmid#233865PurposeMoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2DepositorInsertpTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVMutationSee CommentsPromoterCaMKIIAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRc/RSV Flag MKK3(glu)
Plasmid#14670DepositorAvailable SinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB
Plasmid#86194Purposebeta Globin NMD construct with and without PTC under Bi-directional TET on promoterDepositorUseTet onExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterTet onAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB
Plasmid#78348PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Cdt1
Plasmid#80426PurposeUsed for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.DepositorInsertSpCas9-Cdt1
UseCRISPRTagsCdt1Mutationcodon optimized for humanAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
ExpressionBacterialPromotertetpAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-promoter
Plasmid#21710DepositorInsertROSA26 promoter (Gt(ROSA)26Sor Mouse)
ExpressionMammalianAvailable SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRMT2_pcDNA6.2/EmGFP-Bsd
Plasmid#176953PurposeMammalian expression vector encoding PRMT2 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cluc-RMA
Plasmid#189624PurposeExpresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.DepositorInsertCypridina luciferase fused to Fc
UseAAV and LuciferaseTagsCluc and IgG1 FcExpressionMammalianPromoterhSynAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsmCherryExpressionBacterialAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only