179,521 results
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Plasmid#67935PurposeMammalian expression of mouse vinculin with GFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (Vcl Mouse)
TagsEGFPExpressionMammalianMutationL159P in vinculinPromoterCMVAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-NBL10
Plasmid#69971PurposeCre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusionDepositorInsertNBL10
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterEF1aAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GIV-WT - FLAG
Plasmid#65947PurposeExpression GIV/Girdin in Mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGIV/Girdin (CCDC88A Human)
Tags3xFlagExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
ExpressionBacterialPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase domain
Plasmid#67516PurposeRecruitable human type IV 5-phosphatase domain (214-644)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmRFPMutationINPP5E has C641A to destroy the C-terminal CAAX d…Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequencePromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Prab-3::NLS::GCaMP6s
Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
ExpressionWormPromoterrab-3Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Cyto-CaNAR2
Plasmid#64729PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCaNAR2-NES
TagsCerulean3, Nuclear export signal (NES), and YPetExpressionMammalianPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-TAF1
Plasmid#65395PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTAF1 (TAF1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer P-null replicon
Plasmid#65462Purposecontaining 1.3 units of the P-null HBV genome (subtype ayw)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1.3 units of the P-null HBV genome
ExpressionMammalianAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPC-MYC-hTRF1
Plasmid#64164PurposeRetroviral vector expressing human TRF1 with N-terminal MYC tagDepositorAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SmoM2 (W535L)-pcw107-V5
Plasmid#64628Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMU-PMr
Plasmid#61180PurposeFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPIP2a
TagsmCherryExpressionPlantPromoterAtUBQ10Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJZC116
Plasmid#62344PurposesgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFPDepositorInsertssgRNA + 2x MS2 (wt+f6) binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGCPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-Trastuzumab-IgA1/κ
Plasmid#61888PurposeExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX854
Plasmid#62886PurposeC-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 (aa573-1368)
UseCRISPRTagsFKBP12, NLS SV40, and NLS nucleoplasminExpressionMammalianPromoterCBhAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV5-Flag-HDAC3
Plasmid#63676PurposeMammalian expression of Flag tagged HDAC3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHDAC3 (Hdac3 Mouse)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX853
Plasmid#62885PurposeN-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 (aa 2-573)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianPromoterCBhAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FURIN-bio-His
Plasmid#51755PurposeExpresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFURIN (FURIN Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddRFP A-WW domain-ERK substrate-ddFP B
TagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-pHuji
Plasmid#61555PurposeRed fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHuji
Tags6xHisExpressionBacterialAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorPublicationInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Mito-7
Plasmid#55248PurposeLocalization: Mitochondria, Excitation: 383, Emission: 448DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMito (COX8A Human)
TagsEBFP2ExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCAG-B19G
Plasmid#59921PurposeExpression vector for Rabies virus SAD B19 glycoprotein geneDepositorInsertRabies virus glycoprotein
ExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-EB3-7
Plasmid#55037PurposeLocalization: MT End Binding Protein, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3 (MAPRE3 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
Plasmid#58431PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
ExpressionYeastPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
hph
UseCRISPRPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP104-ChR2-mCherry
Plasmid#58892PurposeAAV-mediated expression of ChR2-mCherry under the GFAP promoterDepositorHas serviceAAV5InsertChR2-mCherry
UseAAVTagsmCherryExpressionMammalianPromoterGFAP104Available sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-dox
Plasmid#58749Purposedoxycycline inducible multicistronic lentiviral gene expression system.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianPromoterTRE2 upstream of minimal CMV promoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dronpa-Lifeact-7
Plasmid#54740PurposeLocalization: Actin, Excitation: 503, Emission: 518DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-Dronpa
ExpressionMammalianAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
AmCyan1-N1
Plasmid#54616PurposeLocalization: N1 Cloning Vector, Excitation: 453, Emission: 486DepositorTypeEmpty backboneTagsAmCyan1ExpressionMammalianAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mT-Sapphire-C1
Plasmid#54545PurposeLocalization: C1 Cloning Vector, Excitation: 399, Emission: 511DepositorTypeEmpty backboneTagsmT-SapphireExpressionMammalianPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEmerald-Fascin-C-10
Plasmid#54094PurposeLocalization: Actin Bundling Protein, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFascin (FSCN1 Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3-RPS6 (pDD1902)
Plasmid#52913Purposecontains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.DepositorAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLucIPZ
Plasmid#53222PurposeConstitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable sinceJune 9, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pVITRO-HPV11 L1L2
Plasmid#52590PurposeExpresses HPV11 L1 and L2 in mammalian cellsDepositorInsertsHPV11 L1
HPV11 L2
ExpressionMammalianAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV phSyn1(S)-FlpO-bGHpA
Plasmid#51669PurposeCan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.DepositorHas serviceAAV RetrogradeInsertFlpO recombinase gene
UseAAVExpressionMammalianPromoterhSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
Tags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Cdc42BPB
Plasmid#50759Purposeexpression of EGFP tagged Cdc42BPBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCdc42BPB (CDC42BPB Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by