164,220 results

1. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Plasmid

   #238237

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS_FtoG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGEN-dPspCas13b-NES-mCherry

   Plasmid

   #233609

   Purpose

   Expression of catalytically-inactive PspCas13b in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGEN-dPspCas13b-4EHP-NES-mCherry

   Plasmid

   #233610

   Purpose

   Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-4EHP-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Expression

   Mutation

   H133A/H1058A

   Promoter

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-Synapto-pHluorin

   Plasmid

   #168510

   Purpose

   Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   Synaptophysin-pHluorin (Syp Mouse)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3544 pMIG Bcl-2

   Plasmid

   #8793

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-DAXX-His

   Plasmid

   #175782

   Purpose

   DAXX protein for bacteria expression system

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NbV5-mCherry

   Plasmid

   #201474

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMitoLOC

    Plasmid

    #58980

    Purpose

    Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    preCOX4 and preSU9

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and MET17

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. OMM-SPLICSs-nFAST

    Plasmid

    #210430

    Purpose

    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    OMM-SPLICSs-nFAST
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    David Root, John Doench

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-CRAC version 1

    Plasmid

    #101245

    Purpose

    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

    Depositor

    Yubin Zhou

    Article

    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

    Insert

    LOV2 and STIM1 cytosolic fragment
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPS2991

    Plasmid

    #8931

    Depositor

    Pamela Silver

    Article

    Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

    Insert

    NDR2 (STK38L Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pAW12.lentiguide.GFP

    Plasmid

    #104374

    Purpose

    GFP vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Syn-PSD95-mCherry

    Plasmid

    #125694

    Purpose

    Exogenous expression of PSD95 (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tau (wt) -VC

    Plasmid

    #87369

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQmod3C-GG

    Plasmid

    #191350

    Purpose

    Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiFCt-2in1-NC

    Plasmid

    #105112

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Use

    Tags

    cYFP-Myc and nYFP-HA

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRRL-TetO-Pax7-IRES-EGFP

    Plasmid

    #109372

    Purpose

    Expresses Dox-inducible Pax7 together with a GFP reporter

    Depositor

    Nenad Bursac

    Article

    Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

    Insert

    Pax7
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMU-GAr

    Plasmid

    #61172

    Purpose

    Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    GmMAN49-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3.1-hMcl-1

    Plasmid

    #25375

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    3' UTR only

    Promoter

    SP6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ALB-bio-His

    Plasmid

    #52176

    Purpose

    Expresses full-length Serum albumin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ALB (ALB Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCI-M2

    Plasmid

    #44170

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Insert

    Influenza A virus M2 matrix gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-EGFP-Rac1-T17N

    Plasmid

    #12982

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 DN (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFZ19

    Plasmid

    #36184

    Depositor

    Daniel Voytas

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

    Type

    Empty backbone

    Use

    Plant t-dna vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    XVE

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid

    #50463

    Purpose

    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAV-GfaABC1D-ERCreER-4x6T

    Plasmid

    #196411

    Purpose

    Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    ERT2-Cre-ERT2
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCherry-Rab11a-7

    Plasmid

    #55124

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge