Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

170,787 results

Showing: 9641 - 9680 of 170787 results

  1. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

    Enlarge

  4. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid
    #140892
    Purpose
    Expresses modified Danio rerio VSP with enhanced phosphatase activity fused with mCherry in mammalian cells.
    Depositor
    Yasushi Okamura
    Article
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Insert
    CiDr-VSP L223F-mCherry (tpte, vsp Ciona intestinalis, Zebrafish)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE)
    Available Since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140892-plasmid-map-sequence-id-292709

    Enlarge

  5. pCEC-red

    Plasmid
    #196040
    Purpose
    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly
    Depositor
    Paola Branduardi
    Article
    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Grna cloning
    Expression
    Yeast
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196040-plasmid-map-sequence-id-386150

    Enlarge

  11. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

    Enlarge

  12. pJB06

    Plasmid
    #190480
    Purpose
    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Cas9
    XylR
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Promoter
    xylR-driven promoter
    Available Since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190480-plasmid-map-sequence-id-374623

    Enlarge

  13. pJB07

    Plasmid
    #190481
    Purpose
    Targeting plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Upstream & Downstream pyrE deletion region
    gRNA
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190481-plasmid-map-sequence-id-372027

    Enlarge

  14. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86556-plasmid-map-sequence-id-176429

    Enlarge

  19. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72323-plasmid-map-sequence-id-137256

    Enlarge

  21. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

    Enlarge

  31. pMRX-IBU-pSu9-HaloTag7-SNAP

    Plasmid
    #217319
    Purpose
    Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.
    Depositor
    Noboru Mizushima
    Article
    Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:
    Insert
    Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag
    Use
    Retroviral
    Tags
    HaloTag and SNAP
    Promoter
    CMV
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    217319-plasmid-map-sequence-id-436845

    Enlarge

  34. pAAV-Syn-iCre

    Plasmid
    #122518
    Purpose
    Cre expression
    Depositor
    Kimberly Ritola
    Insert
    iCre
    Use
    AAV
    Available Since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    122518-plasmid-map-sequence-id-237797

    Enlarge

  37. plex_306-ccdB-zeo

    Plasmid
    #201134
    Purpose
    pLEX_306 plasmid with zeo marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201134-plasmid-map-sequence-id-395510

    Enlarge

Showing: 9641 - 9680 of 170787 results