164,223 results

1. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

   Plasmid

   #141391

   Purpose

   Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-N (N SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMC_mNG2(11)_BSDminus_F2

   Plasmid

   #184164

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

   Plasmid

   #124494

   Purpose

   Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Caspase-1 cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEmerald-TPT1-C-10

   Plasmid

   #54283

   Purpose

   Localization: Microtubule Associated Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   TPT1 (TPT1 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pcDNA3-UGAcS

   Plasmid

   #172135

   Purpose

   mammalian expression plasmid for a UDP-GlcNAc sensor

   Depositor

   Huiwang Ai

   Article

   Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

   Insert

   UGAcS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-N-Puro-mStayGold

   Plasmid

   #227250

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF1a-mEmerald-CAPRIN1

   Plasmid

   #164218

   Purpose

   Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   CAPRIN1 (CAPRIN1 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JB94

    Plasmid

    #128905

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab7ATN-BirA*-HA-MAO (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Miro1 S156E

    Plasmid

    #127589

    Purpose

    Phospho-mimetic of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156E

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pfwB

    Plasmid

    #37329

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221_SLC5A9

    Plasmid

    #132230

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A9 (SLC5A9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJM105: pTac RFP on pCM66T

    Plasmid

    #74746

    Purpose

    pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX165-HiFi Cas9

    Plasmid

    #140563

    Purpose

    Expresses HiFi Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    R691A

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiPGK Hygro DEST H2B-mRuby2

    Plasmid

    #90236

    Purpose

    Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73022

    Purpose

    Luminescent and fluorescent dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JB82

    Plasmid

    #128911

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab10TN-BirA*-HA-MAO (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JB81

    Plasmid

    #128910

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab10QL-BirA*-HA-MAO (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNIFV

    Plasmid

    #111085

    Purpose

    pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors

    Depositor

    David Russell

    Article

    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

    Insert

    empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
    

    Use

    Deleted foamy virus

    Tags

    Expression

    Mutation

    at nt 2334 A changed to T, at nt 2495 A changed t…

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lenti-25xUPRE-minP-EGFP

    Plasmid

    #159668

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xUPRE-minP-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCW-eGFP

    Plasmid

    #162823

    Purpose

    Doxycycline-inducible overexpression of eGFP

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V163A

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPB-CAG-Venus-Akaluc

    Plasmid

    #209768

    Purpose

    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).

    Depositor

    Yohei Hayashi

    Insert

    Venus-Akaluc
    

    Use

    Luciferase; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF0320

    Plasmid

    #141582

    Purpose

    Lentiviral vector for overexpressing the MSLN transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MSLN (MSLN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TCARZ-CMV-GFP-PAmin-GF3-R4A22

    Plasmid

    #203768

    Purpose

    For production of template RNA for use in PRINT, GFP under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTK_TFAP2a_enh_249_Cerulean

    Plasmid

    #130615

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    TFAP2a_enh_249
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223-ACVR1B

    Plasmid

    #23567

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR1B (ACVR1B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pCMV6-AC EJ7-GFP

    Plasmid

    #113617

    Purpose

    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    EJ7-GFP variant of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCVbsd-PPARD-WT

    Plasmid

    #130724

    Purpose

    Vector encoding PPARD cDNA for retroviral transduction

    Depositor

    Till Adhikary

    Article

    Legrand et al Nucleic Acids Res. 2019 Oct 10;47(18):95

    Insert

    PPARD (PPARD Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 29, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pRK5-FLAG-C12orf66

    Plasmid

    #87047

    Purpose

    mammalian expression of C12orf66

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    C12orf66 (C12orf66 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. CAG FLEX BARK D110A p2A mCherry

    Plasmid

    #117694

    Purpose

    CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    Cre/Lox

    Tags

    HA / FLAG

    Expression

    Mutation

    D110A

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pmCherry-C1-mTrim21

    Plasmid

    #105516

    Purpose

    Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. D1433 his3::LYS2 Disruptor Converter

    Plasmid

    #51676

    Purpose

    Yeast marker swap plasmid for converting HIS3 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

    Plasmid

    #63931

    Purpose

    Empty AAV-RAM plasmid w. multiple cloning site (MCS)

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pRAM and TRE

    Available Since

    Dec. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPB-CAG-rtTA-IRES-Hygro

    Plasmid

    #102423

    Purpose

    Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    tetracyclin-transactivator (rtTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lenti-EFS-EGFP-T2A-Bsd

    Plasmid

    #193198

    Purpose

    Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. UKNP1.9.1

    Plasmid

    #97374

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRG217

    Plasmid

    #64531

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge