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pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSIRV-NFAT-eGFP

Plasmid

#118031

Purpose

Fluorescent reporter for NFAT activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

eGFP

Use

Retroviral

Promoter

minimal promoter with NFAT binding site

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TRE-EGFP-EF1a-rtTA

Plasmid

#104454

Purpose

PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

Depositor

Volker Busskamp

Article

Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

Inserts

enhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3

Use

Piggybac

Expression

Mammalian

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19

Plasmid

#50005

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cloning vector

Promoter

lac

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDRGFP

Plasmid

#26475

Purpose

In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

Insert

DRGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#226475

Purpose

Improved microglia-targeted transgene expression

Depositor

Hirokazu Hirai

Article

Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
target sequences for miR-9x4 & miR-129-2-3px4 *2
EGFP

Use

AAV

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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SP-dCas9-VPR

Plasmid

#63798

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-mTagBFP2

Plasmid

#34632

Depositor

Vladislav Verkhusha

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

mTagBFP2

Expression

Bacterial

Mutation

I174A compared to mTagBFP (enhanced photostabilit…

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pSIRV-AP-1-mCherry

Plasmid

#118095

Purpose

Fluorescent reporter for AP-1 activation

Depositor

Peter Steinberger

Article

Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

Insert

mCherry

Use

Retroviral

Promoter

minimal promoter with AP-1 responsive element

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Article

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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EF.STAT3DN.Ubc.GFP

Plasmid

#24984

Purpose

Depositor

Linzhao Cheng

Article

Hillion et al Cancer Research 68, 10121, December 2008

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Y705F--dominant negative

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-Y705F

Plasmid

#74434

Purpose

Expression of STAT3-Y705F

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

Flag

Expression

Mammalian

Mutation

Mutations E16K, Y705F

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Ub-M-GFP

Plasmid

#11938

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-M-GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-tdTomato

Plasmid

#72486

Purpose

Express Luc2 and tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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