4,132 results

1. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB542

   Plasmid

   #27395

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, groESL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1 human cofilin WT

   Plasmid

   #50859

   Purpose

   expresses human cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHM6-alphasynuclein-A53T

   Plasmid

   #40825

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-flpE-puro

   Plasmid

   #20733

   Purpose

   Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   FLPe recombinase
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rab5a-pmCherryC1

   Plasmid

   #27679

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Rab5a (Rab5a Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMVTNT PINK1 C-myc

    Plasmid

    #13314

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    Behnam Nabet, James Bradner

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVHA E2F1

    Plasmid

    #24225

    Purpose

    Mammalian expression of human E2F1 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEM-3z/601

    Plasmid

    #26656

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Insert

    
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FLEX-DTR-GFP

    Plasmid

    #124364

    Purpose

    conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

    Depositor

    Eiman Azim, Thomas Jessell

    Article

    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

    Has Service

    AAV2

    Insert

    DTR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Chicken B-actin

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Use

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pAAV-FLEX-tdTomato

    Plasmid

    #28306

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB_rtTA_BsmBI

    Plasmid

    #126028

    Purpose

    PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSF1389

    Plasmid

    #104962

    Purpose

    Bacterial expression plasmid of bdSENP1 protease

    Depositor

    Dirk Görlich

    Article

    Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

    Insert

    SENP1
    

    Use

    Tags

    His14 tag and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP ZO1

    Plasmid

    #30313

    Depositor

    Alan Fanning

    Article

    Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

    Insert

    Zonula Occludens-1 (TJP1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Unique spliceform*

    Promoter

    Available since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGL3 BRE Luciferase

    Plasmid

    #45126

    Depositor

    Peter ten Dijke, Martine Roussel

    Article

    Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

    Insert

    Id1-BRE
    

    Use

    Luciferase; Bmp/smad transcriptional reporter

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK5-EGFP-Tau AP

    Plasmid

    #46905

    Purpose

    expresses EGFP tagged Tau AP in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau AP (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    all 14 S/P or T/P amino acid residues (T111, T153…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTREtight2

    Plasmid

    #19407

    Depositor

    Markus Ralser

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3.1-pCMV-PfV-GS-Sapphire

    Plasmid

    #116933

    Purpose

    Mammalian vector for expression of the 40nm GEM

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 2XMyc-LRRK2-WT

    Plasmid

    #25361

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge